Laboratories

Prof. dr hab. Zdzisław Świderski - head
Dr hab. Vasyl V. Tkach
Dr hab. Daniel Młocicki

tel. 22-697-89-70, 22-697-89-66

Rysunek1_2_2.jpg

Prof. dr hab. Zdzisław Świderski i doc. dr hab. Vasyl V. Tkach

Rysunek2_1.jpg

Dr Daniel Młocicki

Rysunek3_1.jpg

Doc. Dr hab. Vasyl V. Tkach i Prof. Lena Jarecka

Actual programme of research and scientific activity:

Our laboratory is conducting the extensive comparative studies on: (1) embryogenesis in cestodes and trematodes by means of electron microscopy (TEM and SEM) and cytochemistry; (2) cellular organization of the infective larval stages of parasitic platyhelminths; (3) the post-embryonic development and ultrastructure of their larvae and juvenile forms. Several species selected for our studies are of medical and/or veterinary importance, such as three species of examined schistosomes, taeniid cestode including Echinococcus granulosus and E. granulosus, anoplocephalids, namely Inermicapsifer madagascariensis, Anoplocephaloides dentata, Mosgovoyia ctenoides and Moniezia sp. Obtained results allow us to elucidate mechanism of host infection and biology of parasite transmission and may represent successful contribution towards the fight against the examined parasites. In addition, new data on the gametogenesis, embryonic and post-embryonic development combined with new molecular data may provide useful information on phylogenesis, systematic and evolution of Platyhelminthes.
The Polish school of parasitology (K. Janicki, L. W. Wiśniewski, J. S. Ruszkowski, W. Michajłow, K. Rybicka, L. Jarecka) has a long tradition in this field of research and always significantly contributed towards its development and current progress. Our labolatory from a long period of time maintain close scientific cooperation with parasitologists from Bulgaria, Czech Republic, Spain, Russia, Switzerland, Ukraine, USA and United Kingdom, working in similar field of research.
The results of our research projects are published almost exclusively in the important international journal included on the ISI Master Journal List. They are frequently presented at both local and international conferences and congresses.

5_1.jpg

TEM: General topography of the Gallegoides arfaai egg(Anoplocephalidea). C-egg capsule; IE-inner envelope; OE- outer envelope; ONC-oncosphere; UW-uterine wall;

Achievements:

  • Elucidation and description of embryogenesis in 18 species of tapeworms and 5 species of trematodes belonging to Digenea, including characteristic of cleavage pattern, blastomere differentiation during morpho- and organogenesis of infective larvae of trematodes and cestodes.
  • Comparative analysis of cellular differentiation of egg envelopes, hooks and glands in the oncospheres of cestodes and miracidia of trematodes;
  • Description of the functional ultrastructure of preacetabular and postacetabular penetration glands of cercariae in three species of schistosomes, parasites of man;
  • Elucidation of the action of three selected anthelmintic drugs: Niridazol, Praziquantel and Amoscanate on three species of human schistosomes;
  • Elucidation of the effects of two anthelminthc drugs: Mebendazol, Fembendazol on ultrastructure of the cysts in two species of echinococcus: E. granulosus and E. multilocularis.Elucidation of the effects of two anthelminthc drugs: Mebendazol, Fembendazol on ultrastructure of the cysts in two species of echinococcus: E. granulosus and E. multilocularis;
  • In the framework of KBN grant for the research project: "Molecular basis of systematics of Digenea with particular emphasis on suborder Plagiorchiata" we analyzed the: (1) interrelationships between different taxons of this sub-order and its phylogenetical position among digenetic trematodes; (2) extensive molecular phylogenesis of digenea including about 70 families and 160 genera was established; (3) a great number of new taxons in digeneans was suggested.;
  • Several important questions concerning phylogenesis, systematics and evolution of Digenea were drawn and discussed..

4_1.jpg

Mature egg of G. arfaai

8_1.jpg

TEM: Infective oncosphere of Joyeuxiella echinorhyncoides (Dipylidiidae)

11_1.jpg

J. echinorhyncoides: semi-thin sections of mature eggs

9_1.jpg

Diagram of the infective oncosphere of Mosgovoyia ctenoides (Anoplocephalidea)
BSC - binucleatede subtegumental cell; GC-germinative cells; NC-nerve cells; OT-oncospheral tegumrnt; PG-penetration gland; SC.- somatic cells

7_1.jpg

 TEM: Mature egg of J. echinorhyncoides

6_1_1_1.jpg

TEM: General topograpy of the J. echinorhyncoides egg

Mconcosph_1.jpg

TEM: Infective oncosphere of M. ctenoides

 

Selected publications:

  1. Kedra A.H., Tkach V.V., Swiderski Z., Pawlowski Z. (2001). Intraspecific variability within NADH dehydrogenase subunit 1 sequences of Taenia hydatigena. Parasitology International 50: 145-148.
  2. Swiderski Z., Ndiaye P.I., Miquel J., Tkach V., Marchand B., Chomicz L., Grytner-Ziecina B., Sereda M.J. (2001). Ultrastructural study of the embryonic development of the anoplocephalid cestode Anoplocephaloides dentata, an intestinal parasite of Arvicolidae rodents. II. Differentiation and cellular organisation of the oncosphere. Acta Parasitologica 46(4): 280-292.
  3. Tkach V., Grabda-Kazubska B., Swiderski Z. (2001). Systematic position and phylogenetic relationships of the family Omphalometridae Odening, 1959 (Digenea, Plagiorchiida) inferred from the partial lsrDNA sequences. International Journal for Parasitology 31: 81-85.
  4. Tkach V., Pawlowski J., Mariaux J., Swiderski Z. (2001). Molecular phylogeny of the suborder Plagiorchiata and its position in the system of Digenea. In: Littlewood D.T.J. and Bray R.A. (Eds.), Interrelationships of Platyhelminthes. Taylor & Francis, London, pp. 186-193.
  5. Tkach V.V., Snyder S.D., Swiderski Z. (2001). On the phylogenetic relationships of some members of Macroderoididae and Ochetosomatidae (Digenea, Plagiorchioidea). Acta Parasitologica 46(4): 267-275.
  6. Swiderski Z., Ndiaye P.I., Tkach V., Miquel J., Marchand B., Chomicz L., Sereda M.J. (2001). Ultrastructural study of the embryonic development of the anoplocephalid cestode Anoplocephaloides dentata, intestinal parasite of Arvicolidae rodents. I. Egg envelope formation. Acta Parasitologica 46(3): 171-185.
  7. Moczon T., Swiderski Z. (2002). Schistosoma haematobium: Cytochemistry of the Mehlis' gland and of the ootype wall. Acta Parasitologica 47(4): 280-287.
  8. Swiderski Z., Mackiewicz J.S. (2002). Ultrastructure of spermatogenesis and spermatozoa of the caryophyllidean cestode Glaridacris catostomi Cooper, 1920. Acta Parasitologica 47(2): 83-104.
  9. Swiderski Z., Tkach V.V. (2002). Ultrastructure of embryonic development of Inermicapsifer madagascariensis (Cestoda, Anoplocephalidae) with emphasis on the cellular organisation of the infective eggs. Acta Parasitologica 47(2): 105-120.
  10. Tkach V.V., Lotz J., Swiderski Z., Esteban J.G. (2002). On the systematic position of Ophiosacculus Macy, 1935 (Digenea: Lecithodendriidae), with the erection of the Ophiosacculinae n. subfam. Systematic Parasitology 53: 159-167.
  11. Tkach V.V., Swiderski Z., Drozdz J., Demiaszkiewicz A. (2002). Molecular identification of Echinococcus granulosus from wild European beaver, Castor fiber (L.) from North-Eastern Poland. Acta Parasitologica 47: 173-176.
  12. Tkach V.V., Littlewood D.T.J., Olson P.D., Kinsella J.M., Swiderski Z. (2003). Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Systematic Parasitology 56: 1-15.
  13. Chomicz L., Swiderski Z. (2004). Functional ultrastructure of the oncospheral envelopes of twelve hymenolepidid species with aquatic life cycles. Acta Parasitologica 49(3): 177-181.
  14. Miquel J., Swiderski Z., Mlocicki D., Marchand B. (2004). Ultrastructure of the spermatozoon of the anoplocephalid cestode Gallegoides arfaai (Mobedi and Ghadirian, 1977) Tenora and Mas-Coma, 1978, an intestinal parasite of the wood mouse (Apodemus sylvaticus Linnaeus, 1758). Parasitology Research 94(6): 460-467.
  15. Mlocicki D., Eira C., Zebrowska J., Miquel J., Swiderski Z. (2004). Ultrastructure of the tegument of the anoplocephalid cestode Mosgovoyia ctenoides (Railliet, 1890) Beveridge, 1978. Wiadomosci Parazytologiczne 50(3): 579-585.
  16. Swiderski Z., Brunanska M., Poddubnaya L.G., Mackiewicz J.S. (2004). Cytochemical and ultrastructural study on vitellogenesis in caryophyllidean cestode Khawia armeniaca (Cholodkovski, 1915). Acta Parasitologica 49(1): 16-24.
  17. Swiderski Z., Conn D.B., Miquel J., Mlocicki D. (2004). Fertilization in the cestode Gallegoides arfaai (Mobedi et Ghadirian, 1977) Tenora et Mas-Coma, 1978 (Cyclophyllidea, Anoplocephalidae). Acta Parasitologica 49(2): 108-115.
  18. Swiderski Z., Mackiewicz J.S. (2004). Ultrastructural studies on the cellular organisation of the coracidium of the cestode Bothriocephalus clavibothrium Ariola, 1899 (Pseudophyllidea, Bothriocephalidae). Acta Parasitologica 49(2): 116-139.
  19. Swiderski Z., Miquel J., Agostini S., Conn D.B., Chomicz L., Grytner-Ziecina B. (2004). Differentiation and ultrastructure of the oncospheral envelopes of the cestode Joyeuxiella echinorhyncoides (Sonsino, 1889) (Cyclophyllidea, Dipylidiidae). Acta Parasitologica 49(3): 201-216.
  20. Swiderski Z., Salamatin R.V., Grytner-Ziecina B. Kornyushin V.V., Conn D.B. (2004). Electron microscope study on oncospheral envelope morphogenesis in the dilepidid cestode, Dilepis undula (Schrank, 1788). Acta Parasitologica 49(4): 300-308.
  21. Miquel J., Swiderski Z., Marchand B. (2005). Spermatological characters in the Dipylidiidae Stiles, 1896 (Cestoda, Cyclophyllidea). Acta Parasitologica 50(1): 65-73.
  22. Miquel J., Swiderski Z., Mlocicki D., Eira C., Marchand B. (2005). Ultrastructure of spermatogenesis of the anoplocephalid cestode Gallegoides arfaai (Mobedi and Ghadirian, 1977) Tenora and Mas-Coma, 1978. Acta Parasitologica 50 (2), 132-144.
  23. Mlocicki D., Swiderski Z., Conn D.B., Eira C., Miquel J., Baranowska-Korczyc A. (2005). Ultrastructure of oncospheral hook formation in the cestode Mosgovoyia ctenoides (Railliet, 1890) Beveridge, 1978 (Cyclophyllidea, Anoplocephalidae). Acta Parasitologica 50(2), 124-131.
  24. Mlocicki D., Swiderski Z., Eira C., Miquel J. (2005). An ultrastructural study of embryonic envelope formation in the anoplocephalid cestode Mosgvoyia ctenoides (Railliet, 1890) Beveridge, 1978. Parasitology Research 95(4): 243-251.

dr hab. ANNA ROCKA (WOJCIECHOWSKA)

Anna Rocka

professor IP PAS

Biology, Systematic and Zoogeography of Helminths Laboratory

Tel.: +48 22 751 17 14
E-mail: abroccy@poczta.onet.pl
ResearchGate | Nauka Polska

Research interests:
Morphology, biology of cestodes and nematodes (adults and larval forms), parasites of Antarctic vertebrates.




Ph. D. ZDZISŁAW LASKOWSKI

Zdzisław Laskowski

Department of Biodiversity of Parasites. Laboratory of Biology, Systematic and Zoogeography of Helminths

Tel.: +48 22 697 89 60
E-mail: laskowz@twarda.pan.pl
ResearchGate | Nauka Polska

Research interests:
Molecular systematic, biology and morphology of Antarctic parasites, especially acanthocephalans and digeneans.






Ph. D. WITOLD JEŻEWSKI

Witold Jeżewski

Department of Biodiversity of Parasites. Laboratory of Biology, Systematic and Zoogeography of Helminths

Tel.: +48 22 697 89 60
E-mail: jezw@twarda.pan.pl
ResearchGate | Nauka Polska

Research interests:
Biology, systematic and life cycles of parasites (Digenea, Cestoda, Nematoda, Acanthocephala) of free-living aquatic and terrestrial animals.

prof. Ph. D KRZYSZTOF ZDZITOWIECKI

Krzysztof Zdzitowiecki

professor

Biology, Systematic and Zoogeographyof Helminths Laboratory (retired)

Tel.: +48 22 697 89 81
E-mail: kzdzit@twarda.pan.pl
ResearchGate | Nauka Polska

Research interests:
Parasites of Antarctic mammals, birds, fish and invertebrates, especially acanthocephalans and digeneans (biology, morphology and systematic of Antarctic parasites).

Awards recieved

  • 1977 — Well-Deserved Bronze Cross for participation in the foundation of the Antarctic Station PAS on King George Island.
  • 1987 — Science Secretary of Polish Academy of Sciences Award for scientific achievements (Dr.Hab. (Assistant professorship) dissertation entitled - "Antarctic Acanthocephala").
  • 1990 — Well-Deserved Gold Cross for the whole of scientific activity.



Selected publications:
  1. Hildebrand J., Adamczyk M., Laskowski Z. and Zaleśny G. (2015) Host-dependent morphology of Isthmiophora melis (Schrank, 1788) Luhe, 1909 (Digenea, Echinostomatinae) - Morphological variation vs. molecular stability. Parasites & Vectors 8(1):481
  2. Anna M. Pyziel A. M., Laskowski Z., Höglund J. (2015) Development of a multiplex PCR for identification of Dictyocaulus lungworms in domestic and wild ruminants Parasitology Research. 114:3923-3926
  3. Hildebrand J., Sitko J., Zaleśny G., Jeżewski W., Laskowski Z. (2015) Molecular characteristics of representatives of the genus Brachylecithum Shtrom, 1940 (Digenea, Dicrocoeliidae) with comments on life cycle and host specificity. Parasitology Research. DOI 10.1007/s00436-015-4875-3.
  4. Laskowski Z., Rocka A. 2014. Molecular identification larvae of Onchobothrium antarcticum (Cestoda: Tetraphyllidea) from marbled rockcod, Notothenia rossii, in Admiralty Bay (King George Island, Antarctica). Acta Parasitologica, 59, 767–772.
  5. Laskowski Z., Jeżewski W., Zdzitowiecki K. 2014. Changes in digenean infection of the Antarctic fish Notothenia coriiceps in Admiralty Bay (King George Island) over three decades. Polish Polar Research, 35 (3), 513–520.
  6. Jeżewski W., Zdzitowiecki K., Laskowski Z. 2014. Digenea in notothenioid fish in the Beagle Channel (Magellanic sub-region, sub-Antarctica). Acta Parasitologica, 59, 42–49.
  7. Jeżewski W., Buńkowska-Gawlik K., Hilderbrand J., Perec-Matysiak A., Laskowski Z. 2013. Intermediate and paratenic hosts in the life cycle of Aelurostrongylus abstrusus in natural environment. Veterinary Parasitology, 1998, 401–405.
  8. Zdzitowiecki K., Rocka A., Laskowski Z. 2013. Helminty — mało znani mieszkańcy Antarktyki. Kosmos, 62, 333–340.
  9. Zdzitowiecki K., Laskowski Z. 2013. New data on the occurrence of Acanthocephala in some fish in Admiralty Bay (South Shetland Islands). Acta Parasitologica, 58, 547–550.
  10. Laskowski Z., Jeżewski W., Zdzitowiecki K. 2013. Description of a new opecoelid trematode species from notothenioid fish in the Beagle Channel (sub-Antarctica). Journal of Parasitology, 99, 487–489.
  11. Laskowski Z., Korczas-Abshire M., Zdzitowiecki K. 2012. Changes in acanthocephalan infection of the Antarctic fish Notothenia coriiceps in Admiralty Bay, King Georger island, over 29 years. Polish Polar Research, 33, 99–108.
  12. Jeżewski W., Zdzitowiecki K., Laskowski Z. 2011. Description of Neolepidapedoides subantarcticus sp. nov. (Digenea, Lepocreadiidae) from sub-Antarctic notothenioid fishes. Acta Parasitologica, 56 (3), 305–309.
  13. Laskowski Z., Jeżewski W., Zdzitowiecki K. 2010. New data on the occurrence of Acanthocephala in Antarctic Amphipods. Acta Parasitologica, 55, 161–166.
  14. Laskowski Z., Zdzitowieski K. 2010. Contribution to the knowledge of the infection with Acanthocephala of a predatory Antarctic ice-fish Chaenocephalus aceratus. Polish Polar Research, 31, 303–308.
  15. Jeżewski W., Zdzitowiecki K., Laskowski Z. 2009. Description of a new digenean (Zoogonidae) genus and speciec from sub-Antarctic fish Patagonotothen tessellata. Journal of Parasitology, 95, 1489–1492.
  16. Rokicki J., Rodjuk G., Zdzitowiecki K., Laskowski Z. 2009. Larval ascaridoid nematodes (Anisakidae) in fish from the South Shetland Islands (Southern Ocean). Polish Polar Research, 30, 49–58.
  17. Laskowski Z., Zdzitowiecki K. 2009. Occurrence of acanthocephalans in notothenioid fishes in the Beagle Channel (Magellanic sub-region, sub-Antarctic). Polar Polish Research, 30 (2), 179–186.
  18. Laskowski Z., Rocka A., Zdzitowiecki K., Ozouf-Costaz C. 2008. Occurrence of endoparasitic worms in dusky notothen, Trematomus newnesi (Actinopterygii Nototheniidae), at Adélie Land, Antarctica. Polish Polar Research, 28, 37–42.
  19. Laskowski Z., Jeżewski W., Zdzitowiecki K. 2008. Cystacanth of Acanthocephala in notothenioid fish from the Beagle Channel (sub-Antarctica). Systematic Parasitology, 70, 107–117.
  20. Laskowski Z., Zdzitowiecki K. 2008. New morphological data on the acanthocephalan Hypoechinorhynchus magellanicus Szidat, 1950 (Palaeacanthocephala: Arhythmacanthidae). Systematic Parasitology, 70, 107–117.
  21. Rocka A. 2006. Helminths of Antarctic fishes: Life cycle biology, specificity and geographical distribution. Acta Parasitologica, 51 (1), 26–35.
  22. Laskowski Z., Rocka A., Zdzitowiecki K., Ghigliotti L., Pisano E. 2005. New data on the occurence of internal parasitic worms in the Gymnodraco acuticeps and Cygnodraco mawsoni (Bathydraconidae) fish in the Ross Sea, Antarctica. Polish Polar Research, 26, 37–40.
  23. Zdzitowiecki K., Laskowski Z. 2004. Helminths of an Antarctic fish, Notothenia coriiceps, from the Vernadsky Station (Western Antarctica) on comparison with Admiralty Bay (South Shetland Islands). Helminthologia, 41, 201–207.
  24. Jeżewski W. 2004. Occurrence of Digenea (Trematoda) in two Viviparous species from lakes, rivers and a dam reservoir. Hemlinthologia, 41, 147–150.
  25. Laskowski Z., Zdzitowiecki K. 2004. New morphological data on a sub-Antarctic acanthocephalan, Aspersentis johni (Baylis, 929) (Palaeacanthocephala: Heteracanthocephalidae). Systematic Parasitology, 59, 39–44.
  26. Rocka A. 2004. Nematodes of the Antarctic fishes. Polish Polar Research, 25 (2), 135–152.
  27. Rocka A. 2003. Cestodes of the Antarctic fishes. Polish Polar Research, 24, (3–4), 261–276.

Prof. Mieczysława I. Boguś - head
Emilia Włóka, PhD
Marta Ligęza-Zuber, PhD

Anna Wrońska, PhD
Agata Kaczmarek, M. sc.

Michalina Kazek, M. sc.

tel. 22-679-89-73

 

 

Parasitic fungi are promising candidates for microbiological control of insect pests. Fungi invade insect hosts penetrating their cuticle by a combination of mechanic pressure and enzymatic degradation. Growing fungi release a number of different cuticle-degrading enzymes: proteases, chitinases and lipases. Defense reactions of the host consist mainly of the cellular immune responses effected by the hemolymph cells (hemocytes) which can phagocytose or form multicellular capsules around foreign particles. In rare cases fungal infection stimulates appearance of fungistatic proteins in the insect hemolymph. Most parasitic fungi kill their hosts by some combination of mechanical damage produced by fungal growth and/or nutrient exhaustion. Some fungi rapidly kill insects by releasing toxic metabolites. Entomopathogenic fungi producing mycotoxins attract attention as a potential source of a new generation of insecticides.

 

Hemocyty_Gmell_in_vitro_1_1.jpg

 

 

The hemocytes from Galleria mellonella larvae

Current research:

  1. mechanisms of insect infection by a parasitic fungi :
    • identification of toxic fungal metabolites 
    • function of fungal enzymes in the process of infection
    • influence of culture medium’s ingredient on the insecticide potential 
    • the role of fungal metabolites in the functioning of the insects immune system 
  2. insect defensive reactions to the attack of parasitic fungi:
    • antimicrobial properties of the components of insect cuticle
    • define the role of each class of hemocytes in defense reactions of insects

Applied techniques:

  • gas chromatography with mass spectroscopy (GC / MS), liquid chromatography with mass spectroscopy (LC / MS),
  • light microscopy and fluorescent,
  • flow cytometry,
  • electrophoretic analysis,
  • Western Blot,
  • microbiological testing
 

Gmell_zarażone_Ccoron_.jpg

Galleria mellonella larvae infected with Conidiobolus coronatus

   

Hodowle_Ccoron_2.jpg

Conidiobouls coronatus

 

Hodowle_Ccoron_1.jpg

 

 

Selected publications:

  1. Boguś M.I., Czygier M., Kędra E., Samborski J. (2005). In vitro assessment of the influence of nutrition and temperature on growing rates of five Duddingtonia flagrans isolates, their insecticidal properties and ability to impair Heligmosomoides polygyrus Experimental Parasitology 109:115-123.
  2. Kędra E, Boguś M.I. (2006). The influence of Conidiobolus coronatus on phagocytic activity of insect hemocytes. Journal of Invertebrate Pathology 91: 50-52.
  3. Bania J., Samborski J., Boguś M.I., Polanowski A. (2006). Specificity of an extracellular proteinase from Conidiobolus coronatus and its inhibition by an inhibitor from insect haemolymph. Archives of Insect Biochemistry and Physiology 62:186-196.
  4. Boguś M. I., Kędra E., Bania J., Szczepanik M., Czygier M., Jabłoński P., Pasztaleniec A., Samborski J., Mazgajska J., Polanowski   (2007). Different defense strategies of Dendrolimus pini, Galleria mellonella, and Calliphora vicina against fungal infection. Journal of Insect Physiology 53, 909–922.
  5. Gołębiowski M., Maliński E., Boguś M.I., Kumirska J, Stepnowski P. (2008). The cuticular fatty acids of Calliphora vicina, Dendrolimus pini and Galleria mellonella larvae and their role in resistance to fungal infection. Insect Biochemistry and Molecular Biology 38:619-627.
  6. Gołębiowski M., Boguś M. I, Paszkiewicz M., Stepnowski P. (2010). The composition of the free fatty acids from Dendrolimus pini Journal of Insect Physiology 56:391-397.
  7. Boguś M.I., Czygier , Gołębiowski M., Kędra E., Kucińska J., Mazgajska J., Samborski J., Wieloch W., Włóka E. (2010) Effects of insect cuticular fatty acids on in vitro growth and pathogenicity of the entomopathogenic fungus Conidiobolus coronatus. Experimental Parasitology 125:400-408.
  8. Gołębiowski M., Boguś M.I., Paszkiewicz M., Stepnowski P. (2011). Cuticular lipids of insects as a potential biofungicides: methods of lipids composition analysis. Analytical & Bioanalytical Chemistry 399:3177-3191.
  9. Wieloch , Boguś M.I., Ligęza M., Koszela-Piotrowska I., Szewczyk A. (2011). Coronatin-1 isolated from entomopathogenic fungus Conidiobolus coronatus kills Galleria mellonella hemocytes in vitro and forms potassium channels in black lipid membrane. Toxicon 58:369–379.
  10. Gołębiowski M., Paszkiewicz M., Grubba A., Gąsiewska D., Boguś M.I., Włóka E.,  Wieloch W., Stepnowski P. (2012) Cuticular and internal n-alkane composition of Lucilia sericata larvae, pupae, male and female imagines; application of HPLC-LLSD and GC/MS-SIM. Bulletin of Entomological Research 102:453-460.
  11. Gołębiowski M., Boguś M.I., Paszkiewicz M., Wieloch W., Włóka E., Stepnowski P. (2012). The composition of the cuticular and internal free fatty acids and alcohols from Lucilia sericata males and females. Lipids 47:613–622.
  12. Gołębiowski M., Dawgul M., Kamysz W., Boguś M.I., Wieloch W., Włóka E., Paszkiewicz M., Przybysz E., Stepnowski P. (2012). The antimicrobial activity of the alcohols from Musca domestica. Journal of Experimental Biology 215, 3419-3428.
  13. Gołębiowski M., Cerkowniak M., Boguś M.I., Włóka E., Dawgul M., Kamysz W., Stepnowski P. (2013). Free fatty acids in the cuticular and internal lipids of Calliphora vomitoria and their antimicrobial activity. Journal of Insect Physiology 59,416–429.
  14. Gołębiowski M., Cerkowniak M., Dawgul M., Kamysz W., Boguś M.I., Stepnowski P. (2013). The antifungal activity of the cuticular and internal fatty acid methyl esters and alcohols in Calliphora vomitoria. Parasitology 140, 972-985 doi:10.1017/S0031182013000267.
  15. Gołębiowski M., Cerkowniak M., Boguś M.I., Włóka E., Przybysz E., Stepnowski P. (2013). Developmental Changes in the Composition of Sterols and Glycerol in the Cuticular and Internal Lipids of Three Species of Fly. Chemistry & Biodiversity 10(8):1521-1530.
  16. Gołębiowski M., Urbanek A., Oleszczak A., Dawgul M., Kamysz W., Boguś M.I., Stepnowski P. (2014). The antifungal activity of fatty acids of all stages of  Sarcophaga carnaria  (Diptera: Sarcophagidae). Microbiological Research 169: 279-286.
  17. Gołębiowski M., Cerkowniak M., Urbanek A., Dawgul M., Kamysz W., Boguś , Sosnowska D., Stepnowski P. (2014) Antimicrobial activity of untypical lipid compounds in the  cuticular and internal lipids of four fly species. Journal of Applied Microbiology 116: 269-287.
  18. Gołębiowski M., Sosnowska A., Puzyn T., Boguś M.I., Wieloch W., Włóka E., Stepnowski P. (2014). Application Two-Ways Hierarchic Cluster Analysis for identification similarities between the individual lipid fractions of Lucilia sericata. Chemistry & Biodiversity 11, 733-748.
  19. Gołębiowski M., Cerkowniak M., Urbanek A., Dawgul M., Kamysz W., Boguś M. I., Stepnowski P. (2015). Identification and antifungal activity of the novel organic compounds found in cuticular and internal lipids of medically important flies, Microbiological Research 170, 213–222. http://dx.doi.org/10.1016/j.micres.2014.06.004
  20. Paszkiewicz M., Sikora A., Boguś M.I., Włóka E., Stepnowski P., Gołębiowski M. (2015). Effect of exposure to chlorpyrifos on the cuticular and internal lipid composition of Blattella germanica Insect Science 2015 Jan 6. doi: 10.1111/1744-7917.12200.
  21. Paszkiewicz , Gołębiowski M., Sychowska J., Boguś M.I., Włóka E., Stepnowski P. (2016). The effect of the entomopathogenic fungus, Conidiobolus coronatus, on the composition of cuticular and internal lipids of Blatta orientalis females. Physiological Entomology (w druku)
  22. Gołębiowski M., Cerkowniak M., Boguś M.I., Stepnowski P. (2016). Determination of cuticular and internal fatty acids of Chorthippus brunneus males and females using HPLC- LSD and GC–MS. Biomedical Chromatography (w druku)
  23. Gołębiowski M., Cerkowniak M., Ostachowska A., Naczk A.M., Boguś M.I., Stepnowski P. Effect of Conidiobolus coronatus on the cuticular and internal lipid composition of Tettigonia viridissima males. Chemistry & Biodiversity (w druku)
  24. Gołębiowski M., Ostachowska O., Paszkiewicz M., Boguś M.I., Włóka E., Ligęza-Żuber M., Stepnowski P. (2016). Fatty acids and amino acids of entomopathogenic fungus Conidiobolus coronatus grown on minimal and rich media. Chemical Papers (w druku)

Prof. Bożena Moskwa - head
Prof.Władysław Cabaj
Ph. D. Katarzyna Goździk
Ph. D Justyna Bień
Msc. Aleksandra Cybulska
Msc. Aleksandra Kornacka
Ewa Ryll

tel. 22 697 89 74

 

 

Two main research fields of Laboratory are:

Trichinellosis  - zoonosis which is caused by eating raw or undercooked meat of animals infected with the larvae of a worm called Trichinella. Infection occurs in wild carnivorous (meat-eating) animals but may also occur in domestic pigs and wild boars.


Neosporosis - disease which is caused by a very small, one celled parasite called Neospora canium. This parasite has a worldwide distribution and is now recognized as a common cause of bovine abortion in dairy herds.

larwy1_1.jpg

Larwy Trichinella in muscle

Tri.br3_1.jpg

Larwy Trichinella isolated from the muscle

The distribution of Trichinella species in domestic pigs and in wild animals such as red foxes, wolves and wild boars in different regions of Poland is monitored.
Trichinella larvae have been collected after artificial digestion of the predilection muscle. The method is recommended by International Commission on Trichinellosis. 
Polymerase chain reaction (PCR) analysis have been used to identify larvae at the species level.
It has been found that Trichinella spiralis is the most frequent species in domestic pigs and wild boars. However the mixed infections T. spiralis and T. britovi have been noticed too. In red foxes, the majority of infections are caused by T. britovi.

trich4_1.jpg

Adult worms and new-born larvae isolated from the intestine

justELISA_1.jpg

The prevalence of specific IgG antibodies against T. spiralis in domestic pigs and wild boars is monitored using immunoassay ELISA.

 

Since 2004, the usefulness of ELISA tests in T. spiralis detection in human infections has been studied. Sera from some outbreaks were checked using commercially available test as well as the protocols prepared in our lab. The results revealed that the protocols prepared in our lab detected much more positive sera than commercially available test.

 

Neosporosis - Neospora caninum, an apicomplexan protozoan has a world wide distribution. It caused economical losses due to reproductive failure associated with abortion among cattle. The study is done to check the level of specific antibodies against parasite using the ELISA test kit (IDEXX Lab., Inc., Westbrook, Maine, USA). More than 2500 dams belongs to 11 herds were examined. The prevalence of antibodies varied from 1,5% to 23%.

The cellular and humoral immune responses in pregnant cow during natural long lasting infection of N. caninum were examined. Non infected, pregnant cow was used as a control. Peripheral blood mononuclear cells were stimulated in vitro with Con A, LPS and N. caninum crude antigen. Serum specific IgG level was also examined. The results revealed limited changes in cell-mediated response and slight changes in humoral response in long lasting N. caninum infection in pregnant cow.

IM000023_1.jpg

neo1_1.jpg

Tachyzoites of Neospora caninum in Vero cell

The identity of the parasite in the tissues of naturally infected calves was confirmed by polymerase chain reaction using specific oligonucleotide primers. Additionally, using PCR test, the presence of N. caninum DNA in milk and colostrum from seropositive cows was shown for the first time. The new Polish strain of N. caninum was isolated and it is maintained in vitro (Vero cell culture) and in vivo (gerbils). The Polish isolate was sent to the Moredun Research Institute in Edinburgh (Scotland) for further molecular studies.

 

krowa_1.jpg

Selected publications 2007-2015:

  1. Goździk K., Cabaj W.2007. Characterization of the first Polish isolate of Neospora caninum from cattle. Acta Parasitologica, 52, 295-297.
  2. Moskwa B., Bień J., Goździk K., Cabaj W. 2008. The studies on Neospora caninum DNA detection in the oocytes and embryos collected from infected cows. Veterinary Parasitology, 158, 370-375.
  3. Moskwa B., Bień J., Cabaj W., Korinkova K., Koudela B., Stefaniak J.  2009. The comparison of different  ELISA procedures in detection anti-Trichinella IgG in human infections. Veterinary Parasitology, 159, 312-315. 
  4. Bień J., Moskwa B., Cabaj W.2010. In vitro isolation and identification of the first Neospora caninum isolate from European bison. Veterinary Parasitology, 173, 200-2005.
  5. Goździk K., Wrzesień R., Wielgosz-Ostolska A., Bień J., Kozak M., Cabaj W. 2011. Prevalence of antibodies against Neospora caninum in dogs from urban areas in central Poland. Parasitology Research., 90, 991-996.
  6. Bień J., Moskwa B., Bogdaszewski M., Cabaj W. 2012. Detection of specific antibodies anti-Neospora caninum in fallow deer (Dama dama). Research in Veterinary Sciences, 92, 96-98.
  7. Bień J., Nareaho A., Varmaren P., Goździk K., Moskwa B., Cabaj W., Nyman T., Savijoki K. 2012. Comparative analysis of Excretory-Secretory antigens of Trichinella spiralis and Trichinella britovi Muscle Larvae by Two-dimensional difference Gel electrophoresis and Immunobloting. Proteome Science, 10: 10.
  8. Moskwa B., Goździk K., Bień J., Borecka A., Gawor J., Cabaj W. 2013. First report of Trichinella pseudospiralis in red foxes (Vulpes vulpes) in Poland. Acta Parasitologica, 58, 149-154.
  9. Moskwa B., Goździk K., Bień J., Kornacka A., Cybulska A., Reiterova K., cabaj W. 2014. Detection of antibodies to Neospora caninum in mouse (Alces alces); the first report in Europe. Folia Parasitologica, 61, 34-36.
  10. Moskwa B., Bień J., Goździk K., Cabaj W. 2014. The usefulness of DNA derived from third stage larvae in the detection of Ashwortius sidemi infection in european bison, by simple polymerase chain reaction (PCR). Parasites and Vectors, 7, 215 doi: 10.1186/1756-3305-7-215
  11. Moskwa B., Bień J., Cybulska A., Kornacka A., Krzysiak M., Cencek T., Cabaj W. 2015. The first identification of blood-sucking abomasal nematode Ashworthius sidemii in cattle (Bos taurus) using simple polymerase chain reaction (PCR). Veterinary Parasitology, 211 (1-2), 106,109.

Other selected publications:

  1. Cabaj W., Pozio E., Moskwa B., Malczewski A. 2000.Trichinella britovi and T. spiralis in red foxes (Vulpes vulpes) in Poland. Acta Parasitologica, 45 (4), 340-344.
  2. Wiśniewski M., Cabaj W., Moskwa B., Wędrychowicz H. 2002. The first detection of Neospora caninum DNA in brains of calves in Poland. Acta Veterynaria, 52, 393-401.
  3. Borsuk P., Moskwa B., Pastusiak K., Cabaj W. 2003. Molecular identification of Trichinella spiralis and Trichinella britovi by diagnostic multiprimer large mitochondrial rRNA amplification. Parasitology Research, 91, 374-377.
  4. Moskwa B., Cabaj W., Pastusiak K., Bień J.2003. The suitability of milk in detection of Neospora caninum infection in cows. Acta Parasitologica, 48, 138-141.
  5. Cabaj W., Moskwa B., Pastusiak K., Gill J. 2005. Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland. Veterinary Parasitology, 128, 163-168.
  6. Bruschi F., Locci M.T., Cabaj W., Moskwa B., Castagna B., Kociecka W., Masetti M. 2005. Persistence of reactivity against the 45 kDa glycoprotein in late trichinellosis patients. Veterinary Parasitology, 132, 115-118.
dr hab. Grzegorz Karbowiak, prof. IP PAS- head
dr hab. Mykola Ovcharenko, prof. IP PAS

dr Zofia Bogdaszewska

Msc. Joanna Werszko
Msc. Piotr Wróblewski,
Msc Tomasz Szewczyk

tel. 22-697-89-75

Current research:

  • occurrence, biology, morphology and ultrastructure of Trypanosoma and Trypanoplasma species parasitizing freshwater fish in Poland and Ukraine;
  • microparasites occurring in see fishes from the Mugilidae family;
  • comparative study of the life cycles and ultrastructure of microsporidia parasitizing crustaceans belonging to the Cyclopidae, Daphniidae, Gammaridae families and dipterans of the Chironomidae family;
  • the role of crustaceans and larvae of aquatic insects as a zoonotic reservoir of mirosporidiosis for aquatic and terrestrial hosts;
  • the blood parasites (Trypanosoma, Babesia, Hepatozoon, Bartonella) of small mammals and their vectors (blood sucking arthropods);
  • the role of small rodents and ticks as reservoir and vectors of zoonotic diseases of humans and domestic animals;
  • the structure of zoonotic foci and dependence between their components;
  • participation in the Bison Network. The aim is international cooperation regarding research on European bison;
  • application of molecular biology methods to taxonomy of parasitic Protozoa;

Tcervi_1.jpg

Trypanosoma cervi

International cooperation:
  • Badania mikrosporydiów ryb Morza Czarnego. Umowa o współpracy naukowej z Instytutem Biologii Mórz Południowych NAN Ukrainy (M. Ovcharenko, I. Wita);
  • Mikrosporydia ponto-kaspijskich Amphipoda. Polsko-rumuński projekt (M.Ovcharenko, I.. Wita);
  • Evaluing the effect of an invasive species on local mullet communities in the Mediterranean. Międzynarodowy projekt INTAS ref Nr 03-51-5998 (M. Ovcharenko);
  • Ekologia i epidemiologia gryzoni z rodzaju Apodemus w wybranych środowiskach Polski i Słowacji. Umowa o współpracy z instytutem Parazytologii i Instytutem Zoologii Słowackiej Akademii Nauk (G. Karbowiak);
  • Charakterystyka genetyczna piroplazm z rodzaju Babesia. Współpraca z Centers for Diseases Control and Prevention Atlanta, USA (G. Karbowiak).

Ricidermak_1.jpg

aIxodes ricinus, samica; 

b.Dermacentor reticulatus, samica

Scientific exchange:
G. Karbowiak:

  • internship in the National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA;
  • official cooperation between the Institute of Zoology SAN and the Institute of Parasitology SAN in Koszyce, Slovakia;

M. Ovcharenko:

  • stay in Cavanilles Institute of Biodiversity and Evolutionary Biology, Valencia, Spain;
  • stay in the Institute of Biology, Romanian Academy of Sciences, Bukareszt, Romania.


International visits:

  • Slovak Academy of Sciences- dr B. Petko, dr M. Stanko, dr J. Fričova
  • NAN Ukraine - prof. I. Akimov, dr V. Yurakhno,
  • prof. Neelima Gupta - Universyty Rohikhand in Bareilly, India.

Tcarassi_1.JPG

Trypanosoma carasii

Bmicroti_1.jpg

Babesia microti

TRYPanopLA_1.JPG

Trypanoplasma borelli





 

Selected publications:

  1. Karbowiak G., Rychlik L., Nowakowski W. and Wita I. 2005. Natural infections of small mammals withblood parasites on the borderland of boreal and temperate forest zones. Acta Theriologica 50 (1):31-42

  2. Ovcharenko M. and Wita I. 2005 The ultrastructural study of Nosema artemiae (Codreanu, 1957)(Microsporidia:Nosematidae). Acta Protozooogica. 44:33

  3. Ovcharenko M. Wita I. and Czaplińska U. 2005. Ultrastructural data on Gurleya orchestiae Ovcharenko et Kurandina, 1987 (Microsporidia, Gurleyidae). Acta Parasitologica 50 (1):90-93.

  4. Karbowiak G., 2004. Zoonotic reservoir of Babesia microti in Poland. Polish Journal of Microbiology, 53, Suppl., 61-65.

  5. Karbowiak G., Wita I., 2004. Trypanosoma (Herpetosoma) grosi kosewiense subsp. n., the parasite of the yellow-necked mouse Apodemus flavicollis (Melchior, 1834). Acta Protozoologica, 43 (2), 173-178.

  6. Karbowiak G., Wita I., Czaplińska U., 2004. Protozoan parasites in blood of social vole (Microtus socialis) in"Askania-Nova" reserve, Ukraine. Vestnik Zoologii, suppl. N, 18: 186-187.

  7. Karbowiak G., Wita I., Czaplińska U., Slemenda S., Pieniazek N.J., 2004. Canine babesiosis in Poland. Wiadomości Parazytologiczne, 50 (suppl.), 53-54.

  8. Madej G., Karbowiak G., Buczek A., 2004. Morphological anomaly in Laelaps agilis C.L. Koch, 1836 sensu Karg 1971 (Mesostigmata, Acar) collected from nature. In: Buczek A., Błaszak Cz, (ed.), Stawonogi. Interakcje pasożyt-żywiciel. Liber, Lublin, 253-257.

  9. Karbowiak G., Izdebska J.N., Czaplińska U., Wita I., 2003. Przypadki zimowania kleszczy z rodziny Ixodidae na żywicielach w Puszczy Białowieskiej. W: Buczek A., Błaszak Cz. (red. ) Stawonogi i żywiciele. Liber, Lublin, 77-82.

  10. Ovcharenko N. A. 2003. New and modified methods for studying Microsporidia of aquatic animals Hydrobiological Journal, 39 (1), 2003:28 - 38 (English version of Journal, published by Begel House Publishers, USA).

  11. Ovcharenko M. and Wita I. 2003. The ultrastructural study of Microsporidium kosewiense sp. n Chironomidae). Vestnik Zoologiii, 37 (6):41-51.

  12. Wita I., Karbowiak G., Czaplińska U. 2003. Trypanosoma (Herpetosoma microti Laveran aet Pettit, 1909 in the social vole, Microtus socialis (Pallas, 1771) from Ukraine. Acta Parasitologica 48(3), 155-162.

Prof. dr hab. Halina Wędrychowicz - head

Dr inż. Agnieszka Wesołowska

Dr inż. Anna Zawistowska-Deniziak

Dr Luke J. Norbury

Dr inż. Katarzyna Basałaj

Dr inż. Kamil Januszkiewicz

mgr inż. Alicja Sielicka
mgr inż. Przemysław Wilkowski

tel.  22-697-89-82  tel.  22-697-89-61 

Current research:

The Laboratory of Molecular Parasitology aims to discover and implement novel strategies to control parasitic diseases, such as fasciolosis, that affect the health of millions animals and humans worldwide.
We employ cutting-edge research in genomics, cell biology, immunology, epidemiology and biochemistry to discover new ways to combat parasitic disease. The Laboratory has a distinguished tradition of gastrointestinal helminth research, especially as related to vaccine development.

Achievements:

We have tested cDNAs encoding Fasciola hepatica vaccine candidates including glutathione S- transferase and cystein protease in laboratory (rats) and ruminant (sheep) hosts. These enzymes have been expressed in bacteria and/or baculovirus as well as plant systems and have been also tested for vaccine efficacy in rats and sheep using intranasal, oral and intramuscular routes of the antigen administration. We have found that intranasal vaccination may be feasible with both cDNA and protein forms of the cysteine proteinase.

Achievements:

We have tested cDNAs encoding Fasciola hepatica vaccine candidates including glutathione S- transferase and cystein protease in laboratory (rats) and ruminant (sheep) hosts. These enzymes have been expressed in bacteria and/or baculovirus as well as plant systems and have been also tested for vaccine efficacy in rats and sheep using intranasal, oral and intramuscular routes of the antigen administration. We have found that intranasal vaccination may be feasible with both cDNA and protein forms of the cysteine proteinase.

In the Laboratory of Molecular Parasitology techniques such as ELISA, flow cytometry, Real-Time PCR, cloning and recombinant expression, Southern and Western blotting and various types of chromatography (gel filtration, reversed-phase, ion exchange and affinity) are applied routinely.

Projects:

International:

NCN HARMONIA 2012/06/M/NZ6/00115 - Assessment of protective immunity against fasciolosis through vaccination of sheep with a multivalent stage-specific recombinant protein vaccine.

 

Design of Effective and sustainable control strategies for LIVER fluke in Europe ("DELIVER") (6 PR, Contract no.: FOOD-CT-200X-023025)

 

National:

NCN OPUS 2012/05/B/NZ6/00777 - Monoclonal recombinant antibodies for the characterisation of important parasite molecules.

 

NCN N N304 156340 - Analysis of the reproductive potential of the intermediate host, protein profile and immunomodulatory properties of excretory/secretory products  from liver fluke (Fasciola hepatica) strain present in bison from Biolowieski National Park

 

FNP INTER V, 2015. 

NCN N N303 321337 The assessment of immunomodulatory properties of novel Fasciola hepatica newly excysted juvenile cysteine proteases.

 

  1. Selected publications:
  2. Wiśniewski M, Jaros S, Bąska P, Cappello M, Długosz E, Wędrychowicz H, Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection. Exp. Parasitol. 133, 472-82.
  3. Wesołowska A, Zawistowska-Deniziak A, Norbury LJ, Wilkowski P, Januszkiewicz K, Pyziel AM, Zygner W, Wędrychowicz H, Immune responses in rats and sheep induced by a DNA vaccine containing the phosphoglycerate kinase gene of Fasciola hepatica and liver fluke infection. Acta Parasitol. 61, 212-220.
  4. Toczyłowska-Mamińska R, Kloch M, Zawistowska-Deniziak A, Bala A, 2016. Design and characterization of novel all-solid-state potentiometric sensor array dedicated to physiological measurements. Talanta 159, 7-13.
  5. Długosz E, Wasyl K, Klockiewicz M, Wiśniewski M, 2015. Toxocara canis mucins among other excretory-secretory antigens induce in vitro secretion of cytokines by mouse splenocytes. Parasitol Res. 114, 3365–3371.
  6. Januszkiewicz K, Norbury LJ, Wilkowski P, Zawistowska-Deniziak A, Wesołowska A, Wędrychowicz H, 2015. Variations in cercarial production and the level of in vitro activation of metacercariae of two different isolates of Fasciola hepatica. Acta Parasitol. 60, 509-514.
  7. Wędrychowicz H, 2015. Antiparasitic DNA vaccines in 21st century. Acta Parasitol. 60, 179-189.
  8. Zawistowska-Deniziak A, Wasyl K, Norbury LJ, Wesołowska A, Bień J, Grodzik M, Wiśniewski M, Bąska P, Wędrychowicz H, 2013. Characterization and differential expression of cathepsin L3 alleles from Fasciola hepatica. Mol. Biochem. Parasitol. 190, 27-37.
  9. Wesołowska A, Jaros S, Norbury LJ, Jaros D, Zygner W, Wędrychowicz H, 2013. Microarray analysis of rat immune responses to liver fluke infection following vaccination with Fasiola hepatica phosphoglycerate kinase. Exp. Parasitol. 134, 33-38.
  10. Bąska P, Wiśniewski M, Krzyżowska M, Długosz E, Zygner W, Górski P, Wędrychowicz H, 2013. Molecular cloning and characterisation of in vitro immune response against astacin-like metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum. Exp. Parasitol. 133, 472-82.
  11. Zygner W, Gójska-Zygner O, Wesołowska A, Wędrychowicz H, 2013. Urinary creatinine to serum creatinine ratio and renal failure index in dogs infected with Babesia canis. Acta Parasitol. 58, 297-303.
  12. Siwińska A, Bąska P, Daniłowicz-Luebert E, Januszkiewicz K, Wędrychowicz H, Capello M, Wiśniewski M, 2013. Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins. Acta Parasitol. 58, 112-118.
  13. Wasyl K, Zawistowska-Deniziak A, Bąska P, Wędrychowicz H, Wiśniewski M, 2013. Molecular cloning and expression of the cDNA sequence encoding a novel aspartic protease from Uncinaria stenocephala. Exp. Parasitol. 134, 198-206.
  14. Bąska P, Norbury LJ, Wiśniewski M, Januszkiewicz K, Wędrychowicz H, 2013. Excretory/secretoty products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells. Acta Parasitol. 58, 215-217.
  15. Wesołowska A, Norbury LJ, Januszkiewicz K, Jedlina L, Jaros S, Zawistowska-Deniziak A, Zygner W, Wędrychowicz H, 2013. Evaluation of the immune response of male and female rats vaccinated with cDNA encoding a cysteine proteinase of Fasciola hepatica (FhPcW1). Acta Parasitol. 58, 198-206.
  16. Norbury LJ, Januszkiewicz K, Smooker PM, 2012. Fasciola: parasite biologu, disease and control. In: Parasites: ecology, diseases and management (Ed. Gilmar S. Erzinger) Nova Science Publishers, 103-166.
  17. Długosz E, Cendrowski J, Bąska P, Siwińska A, Wędrychowicz H, Wiśniewski M, 2012. Analysis of IL-2 production in hookworm infected hamsters using generated polyclonal antibodies. Bull. Vet. Inst. Pulawy 56, 37-42.
  18. Zygner W, Gójska-Zygner O, Norbury LJ, Wędrychowicz H, 2012. Increased AST/ALT ratio in azotaemic dogs infected with Babesia canis. Pol. J. Vet. Sci. 15, 483-486.
  19. Wesołowska A, Gajewska A, Smaga-Kozłowska K, Kotomski G, Wędrychowicz H, 2012. Effect of Fasciola hepatica proteins on the functioning of rat hepatocytes. Parasitol. Res. 110, 395-402.
  20. Jaros S, Wesołowska A, Jaros D, Zygner W, Wędrychowicz H, 2011. Molecular cloning and characterisation of phosphoglycerate kinase from Fasciola hepatica. Bull. Vet. Inst. Pulawy 55, 417-422.
  21. Goździk K, Wrzesień, Wielgosz-Ostolska A, Bień J, Kozak-Ljunggren M, Cabaj W, 2011. Prevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland. Parasitol Res. 108, 991-996.
  22. Jedlina L, Kozak-Ljunggren M, Wędrychowicz H, 2011. In vivo studies of the early, peritoneal, cellular and free radical response in rats infected with Fasciola hepatica by flow cytometric analysis. Exp. Parasitol. 128, 294-297.
  23. Jaros S, Jaros D, Wesołowska A, Zygner W, Wędrychowicz H, 2010. Blocking Fasciola hepatica energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase. Vet. Parasitol. 172, 229-237.
  24. Kozak M, Wędrychowicz H, 2010. The performance of a PCR assay for field studies on the prevalence of Fasciola hepatica infection in Galba truncatula intermediate host snails. Vet. Parasitol. 168, 25-30
  25. Jaros S, Zygner W, Januszkiewicz K, Jaros D, Wędrychowicz H, 2010. Molecular cloning and biochemical characterization of FhPcW1, a new isoform of cathepsin L from adult Fasciola hepatica. Bull. Vet. Inst. Pulawy 54, 585-590.
  26. Zygner W, Górski P, Wędrychowicz H, 2009. New localities of Dermacentor reticulates tick (vector of Babesia canis) in central and eastern Poland. Pol. J. Vet. Sci. 12, 549-555.
  27. Zygner W, Górski P, Wędrychowicz H, 2009. Detection of the DNA of Borrelia afzelii, Anaplasma phagocytophilum and Babesia canis in blood samples from dogs in Warsaw. Vet. Rec. 164, 465-467.
  28. Kęsik M, Jedlina-Panasiuk L, Kozak-Cięszczyk M, Płucienniczak A, Wędrychowicz H, 2007. Enternal vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1). Vaccine 4, 3619-28.
  29. Wędrychowicz H, Kęsik M, Kaliniak M, Kozak-Cięszczyk M, Jedlina-Panasiuk L, Jaros S, Płucienniczak A, 2007. Vaccine potential of inclusion bodies containing cysteine proteinase of Fasciola hepatica in calves and lambs experimentally challenged with metacercariae of the fluke. Vet. Parasitol. 147, 77-88.
dr hab. Jakub Gawor, professor IP PAS - head
tel. 22 697 89 68
ko___1_1.jpglarwa_gza_1.jpgjaja_s__upk._1.jpg    

Research activities:

  • diagnosis of parasite infections in domestic animals (ruminants, horses, dogs and cats) based on faecal examination for helminth eggs and larvae as well as necropsy;

  • prevalence of Echinococcus multilocularis and Trichinella spp. in wild animals with focus on red foxes;

  • contamination of environment with infective stages of Toxocara spp. with physico-chemical and molecular methods;

  • molecular diagnosis of human and animal toxocarosis;

  • studies on helminths in horses with emphasis on strongyle parasites (Strongylidae).

  Jajo_glisty_ko__skiej_wyizolowane_z_ka__u_1.JPG

 

 

Current research:

  • diagnosis of Toxocara canis and T. cati in dogs and cats and contamination of environment (parks, playgrounds, sand boxes) with Toxocara eggs;

  • dependence of clinical cases of toxocarosis in children upon the prevalence of Toxocara spp. in dogs and cats and contamination of environment with Toxocara eggs in central Poland;

  • prevalence of Echinococcus multilocularis in red foxes in Poland;

  • studies on intermediate hosts of E.multilocularis in Poland;

  • prevalence of Trichinella spp. infection in red foxes; evaluation of anthelmintics in horses;

  • studies on anthelmintic resistance in riding horses.





  Osobnik_Echinococcus_multiloculars_1.JPG

Ongoing research projects:

  • EchinoRisk - "Risk Assessment and Prevention of Alveolar Echinococcosis" (QLK2-CT-2001-01995), 5 Program of EU, 2001-2004. Principal Investigator: prof. dr hab. Andrzej Malczewski;

  • "Dependence of clinical cases of toxocarosis in children upon the prevalence of Toxocara spp. in dogs and cats and contamination of the environment with Toxocara eggs", financed by Polish State Committee for Scientific Research, (3PO5D 074 23) - 2002-2005. Principal Investigator: dr Jakub Gawor;

  • "Environmental, clinical and molecular studies on toxocarosis in children", financed by PSCSR, (2 PO5E 015 27) - 2004-2007. First senior staff members: dr Anna Borecka, dr Jakub Gawor;

  • "Studies on intermediate hosts of tapeworm Echinococcus multilocularis", financed by PSCSR (2P05 D 020 27) - 2004-2007. Principal Investigator: prof. dr. Andrzej Malczewski.

  toxocara_1.jpg
     
     
     
     
    larwa_s__upk._1_1.jpg

 

       

Selected publications:

  1. Gawor J., Malczewski, Rocki B. 2004. Echinococcus multilocularis - niebezpieczny dla człowieka tasiemiec lisów rudych (Vulpes vulpes). Medycyna Wet. 60, 349-351.PDF
  2. Gawor J., Malczewski A., Rocki B., Malczewska M. Borecka A. 2004. Badania nad występowaniem tasiemca Echinococcus multilocularis u lisów rudych w Polsce. Medycyna Wet. 60, 489-491. PDF
  3. Gawor J., Malczewski, Stefaniak J., Nahorski W., Paul M., Kacprzak E., Myjak P. 2004. Zagrożenie bąblowicą wielojamową (alweokokozą) dla ludzi w Polsce. Przegląd Epidemiol., 58, 459-65. PDF
  4. Gawor J., Malczewski A., Malczewska M. 2005. Lis rudy żywicielem tasiemca Echinococcus multilocularis w Polsce. Magazyn Wet., 14, 34-36.
  5. Gawor J, Borecka A. 2004. The contamination of the environment with Toxocara eggs in Mazowieckie voivodship as a risk of toxocarosis in children. Wiad. Parazytol. 50, 237-241. Abstract
  6. Borecka A. 2004. Differentiation of Toxocara spp. eggs isolated from the soil by PCR - linked RFLP. Helminthologia, 41, 185-187. PDF
  7. Gawor J., Malczewski A. 2005. Tasiemiec wielojamowy występujący u lisów jako przyczyna niebezpiecznej choroby odzwierzęcej. Kosmos - Problemy Nauk Biologicznych, 54, 89-94. PDF
  8. Gawor J., Kharchenko V. 2005. Aktualna systematyka małych słupkowców (Cyathostominae) występujących u koni. Medycyna Wet., 61, 1344-1346. PDF
  9. Gawor J., Kita J. 2006. Uwagi praktyczne na temat odrobaczania koni. Życie Wet., 81, 753-756. PDF
  10. Gawor J., Kornaś S., Kharchenko V., Nowosad B., Skalska M. 2006. Pasożyty jelitowe zagrożeniem zdrowia koni w różnych warunkach chowu. Medycyna Wet., 62, 331-334. PDF
  11. Dubinsky P., Malczewski A., Miterpakova M., Gawor J., Reiterova K. 2006. Echinococcus multilocularis in the Carpathian regions of Poland and the Slovak Republic. J. Helminthol., 80, 243-247. Abstract
  12. Gawor J. 2007. Konina jako jedno z potencjalnych źródeł zagrożenia ludzi włośnicą. Życie Wet., 82, 611-614. PDF
  13. Borecka A., Gawor J., Malczewska M., Malczewski A. 2007. Występowanie tsiemca Echinococcus multilocularis u lisów rudych na terenie centralnej Polski. Medycyna Wet., 63, 1333-1335. PDF
  14. Traversa D., Iorio R., Klei T.R., Kharchenko V., Gawor J., Otranto D., Sparagano O.A. 2007. New method for simultaneous species-specific identification of equine strongyles (Nematoda, Strongylida) by reverse line blot hybridisation. J. Clin. Microbiol., 45, 2937-2942. Abstract
  15. Borecka A., Gawor J. 2008. Modification of gDNA extraction from soil for PCR designed for the routine examination of soil samples contaminated for Toxocara spp. eggs. J. Helminthol., 82, 119-122. Abstract
  16. Borecka A., Gawor J., Niedworok M., Sordyl B. 2008. Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unquiculatus). Helminthologia, 45, 147-149. PDFPDF
  17. Borecka A., Gawor J., Malczewska M., Malczewski A. 2008. Occurrence of Echinococcus multilocularis in red foxes (Vulpes vulpes) in southern Poland. Helminthologia, 45, 24-27. PDF
  18. Malczewski A., Gawor J., Malczewska M. 2008. Infection of red foxes (Vulpes vulpes) with Echinococcus multilocularis during the years 2001-2004 in Poland. Parasitol. Res., 103, 501-505. Abstract
  19. Gawor J., Borecka A., Malczewski A. 2008. Zarażenie lisów bąblowcem wielojamowym (Echinococcus multilocularis) jako potencjalne zagrożenie dla ludzi w Polsce. Życie Wet., 83, 24-27. PDF
  20. Gawor J., Borecka A., Żarnowska H., Marczyńska M., Dobosz S. 2008. Environmental and personal risk factors for toxocariasis in children with diagnosed disease in urban and rural areas of central Poland. Vet. Parasitol., 155, 217-222. Abstract
  21. Gawor J., Borecka A., Dobosz S., Marczyńska M., Żarnowska-Prymek H., Trzebicka A., Juszko J. 2008. Toksokaroza - niebezpieczna choroba odzwierzeca - epidemiologia, klinika, diagnostyka i zagrożenia dla dzieci. Życie Wet., 83, 655-658. PDF
  22. Niedworok M., Sordyl B., Borecka A., Gawor J., Małecka-Panas E. 2008. Ocena eozynofilii, stężenia immunoglobuliny E i eozynofilowego białka kationowego podczas leczenia toksokarozy. Wiad. Parazytol., 54, 225-230. PDF
  23. Żarnowska H., Borecka A., Gawor J., Marczyńska M., Dobosz S., Basiak W. 2008. A serological and epidemiological evaluation of risk factors for toxocariasis in children in central Poland. J. Helminthol., 82, 123-127. Abstract
  24. Malczewski A., Borecka A., Malczewska M., Gawor J. 2008. Próba wykrycia żywicieli pośrednich tasiemca Echinococcus multilocularis na terenie Polski. Wiad. Parazytol., 54, 137-141. PDF
  25. Borecka A., Gawor J., Malczewska M., Malczewski a. 2009. Prevalence of zoonotic helminth parasites of the small intestine in red foxes from central Poland. Medycyna Wet., 65, 33-35. PDF
  26. Kornaś S., Gawor J., Cabaret J., Molenda K., Skalska M., Nowosad B. 2009. Morphometric identification of equid cyathostome (Nematoda: Cyathostominea) infective larvae. Vet. Parasitol., 162, 290-294. Abstract
  27. Kornaś S., Skalska M., Nowosad B., Gawor J., Kharchenko V., Cabaret J. 2009. Occurrence of strongyles (Strongylidae) in horses from small farms on the basis of necropsy. Polish J. Vet. Sci., 12, 225-230. Abstract
  28. Slivinska K., Gawor J., Jaworski Z. 2009. The gastro-intestinal parasites in yearlings of wild Polish primitive horses from the Popielno Forest Reserve, Poland. Helminthologia, 46, 9-13. PDF
  29. Borecka A., Gawor J., NIedworok M., Sordyl B. 2010. Częstość występowania jaj inwazyjnych Toxocara spp. w środowisku przydomowym dzieci ze zdiagnozowaną toksokarozą w woj. łódzki. Wiad. Parazytol., 56, 141-144. PDF
  30. Gawor J. 2010. Powstanie Polskiej Sekcji Europejskiego Stowarzyszenia ds. Parazytoz Zwierząt Towarzyszących (ESCCAP Polska). Życie Wet., 85 (7), 620-621.nt
  31. Gawor J. 2010. Odrobaczanie koni - konieczność, czy zbędny wydatek? Hodowca i Jeździec,27, 110-113.
  32. Gawor J. 2011. Włośnica u dzików i zwierząt drapieżnych rosnącym zagrożeniem dla ludzi w Polsce. Życie Wet., 86, 806-809. PDF
  33. Gawor J. 2011. Potencjalne czynniki ryzyka zachorowania na alweolarną echinokokozę dla ludzi w Polsce. Przegląd Epidemiol., 65, 465-470. PDF
  34. Gawor J. 2012. Pasożytnicze zagrożenia dla ludzi i zwierząt. Cz. I. Toksoplazmoza i giardioza. Magazyn Wet., 21, 654-656.
  35. Gawor J. 2012. Pasożytnicze zagrożenia dla ludzi i zwierząt. Cz. II. Bąblowica wielojamowa i włośnica. Magazyn Wet., 21, 921-925.
  36. Zygner W., Gawor J., Karamon J. 2012. Koty polujące jako potencjalne zagrożenie dla ludzi inwazją Echinococcus multilocularis. Czy należy zalecać ich profilaktyczne odrobaczanie? Życie Wet., 87 (11), 937-939. PDF
  37. Gawor J. 2013. Tasiemczyce u koni - trudny problem diagnostyczny. Życie Wet., 88 (7), 581-582. PDF
  38. Borecka A., Gawor J., Zięba F. 2013. A survey of intestinal helminths in wild carnivores from the Tatra National Park, southern Poland. Ann. Parasitol., 59 (4), 169-172. PDF
  39. Gawor J. 2013. Trichinella pseudospiralis - nowy gatunek włośnia w środowisku leśnym w Polsce. Czy wzrasta zagrożenie włośnicą dla ludzi? Życie Wet., 88 (12), 1053-1054. PDF
  40. Slivinska K., Wróblewski Z., Gawor J. 2013. Occurrence of gastro-intestinal parasites in Polish primitive horses from the Roztocze National Park, Poland. Vestnik Zool., 47, 497-505.
  41. Gawor J. 2014. Podstawowe zasady zwalczania inwazji pasożytów jelitowych u zwierząt. Magazyn Wet., 23, 62-64.
  42. Gawor J. 2014. Podstawowe metody diagnostyki koproskopowej pasożytów jelitowych u zwierząt. Magazyn Wet., 23, 166-171.
  43. Frączyk M., Gawor J. 2014. Angiostrongylus vasorum - nicień płucny, nowe zagrożenie dla psów w Polsce. Życie Wet., 89, 124-125. PDF
  44. Frączyk M., Gawor J. 2014. Aelurostrongylus abstrusus - nicień płucny kotów - nowe zagrożenie. Magazyn Wet., 23, 1147-1149. 
  45. Gawor J., Borecka A., Marczyńska M., Dobosz S., Żarnowska-Prymek H. 2015. Risk of human toxocarosis in Poland due to Toxocara infection in dogs and cats. Acta Parasitol., 60(1), 99-104.
  46. Gawor J., Borecka A. 2015. Risk of soil-transmitted helminth infections on agritourism farms in central and eastern Poland. Acta Parasitol., 60(4), 716-720.
  47. Gawor J., Marczyńska M. 2015. Zagrożenie ludzi zoonotycznymi geohelmintami w środowisku miejskim i wiejskim w Polsce. Ryzyko toksokarozy. Medycyna Wet., 71(9), 529-532.

Head: prof. dr hab. Aleksander W. Demiaszkiewicz,
Dr Anna M. Pyziel - adiunkt,
Lek. wet. Katarzyna Filip - assistant trainee,
Teresa Sitek - technician

tel. +48 22 697 89 67


Current research:

  • The subject of work of the Laboratory are investigations on the parasites of the European bison, cervids and beavers, their distribution, morphology, systematic, biology, differential diagnosis and also on the epidemiology, pathology and treatment of parasitoses provoked by them. The forest environment as a source of parasites for humans and animals is one of the most important problems undertaken in the laboratory.
  • The current topics of research are gastrointestinal nematodes from the family Trichostongylidae, lung nematodes from the family Protostrongylidae and tissue nematodes from the subfamily Onchocercinae. Furthermore, the dispersion of ashworthiosis of domestic and wild ruminants, the epidemiology of elaphostrongylosis of Cervidae and the threat it creates for domestic animals and people are investigated. Our research also concerns monitoring the status of helminth infections in the European bison in the Białowieża Forest and in cervids under farm breeding conditions.


Projects:

  • „Molecular analysis of lungworms from the genus Dictyocaulus of free-roaming wild ruminants from Poland”. „Mobility Plus” - III edition, No 1105/MOB/2013/0, 2014-2015. Head of the project: dr Anna M. Pyziel. Location: SLU, Uppsala, Sweden. Mentor: Prof. Johan Höglund.


Achievements:

  • elaboration of the helminth fauna of Cervidae in Poland;
  • investigations on the forming of the helminth fauna of the European bison during 45 years of the restitution of this species;
  • detection and description of the genetic polymorphism of the nematodes from the subfamily Ostertagiinae;
  • elaboration of the theory of the parallel evolution of Nematodes (Trichostrongylidae) and their hosts (Cervidae);
  • investigations on species composition, morphology and differential diagnosis of tissue nematodes from the subfamily Onchocercinae in wild and domestic ruminants in Poland;
  • description of the morphology of eggs, invasive larvae and adults of the nematode Ashworthius sidemi;
  • statement of the endangerment of domestic animals by parasitoses of wildlife (parafasciolopsosis, elephostrongylosis, ashworthiosis);
  • description of clinical elaphostrongylosis of domestic ruminants.

The Laboratory of Parasitoses of Wild Animals can do service diagnostic examinations towards parasitical diseases of wild animals and coproscopic examinations of cervids in farm breeding conditions.

 

 

Selected publications:

  1. Dróżdż J. 1995. Polimorphism in the Ostertagiinae Lopez-Neyra, 1947 and commants on the systematics. Systematic Parasitology, 32, 91-99.
  2. Demiaszkiewicz AW. 1995. Studies on tissue nematodes from subfamily Onchocercinae Leiper, 1911 in free living and domestic ruminants in Poland. Rozprawy Naukowe  i Monografie, Wydawnictwo SGGW, Warszawa, 1-104 (in Polish).
  3. Dróżdż J, Malczewski A, Demiaszkiewicz AW. Lachowicz J. 1997. The helminthofauna of farmed deer (Cervidae) in Poland. Acta Parasitologica, 42, 225-229.
  4. Dróżdż J, Demiaszkiewicz AW, Lachowicz J. 1998. Ashworthius sidemi (Nematoda, Trichostrongylidae) a new parasite of the European bison Bison bonasus (L.) and the question of indepedence of A. gagarini. Acta Parasitologica, 43, 75-80.
  5. Demiaszkiewicz AW, Dróżdż J, Lachowicz J. 2001. Occurrence of microfilariae from subfamily Onchocercinae in red and roe deer in Bieszczady Mountains. Helminthologia,  38, 111-113.
  6. Dróżdż J, Demiaszkiewicz AW, Lachowicz J. 2001. Exchange of gastrointestinal nematodes between roe and red deer (Cervidae) and European bison (Bovidae) in the Bieszczady Mountains (Carpathians, Poland). Acta Parasitologica, 47, 314-317.
  7. Tkach VV, Świderski Z, Dróżdż J, Demiaszkiewicz A. 2002. Molecular identification of Echinococcus granulosus from wild European beaver, Castor fiber (L.) from North-Eastern Poland. Acta Parasitologica, 47, 173-176.
  8. Demiaszkiewicz AW, Dróżdż J, Lachowicz J, Bielecki W. 2003. Experimental infection of cattle with invasive larvae of Elaphostrongylus cervi. Medycyna Weterynaryjna, 59, 639-642 (in Polish).
  9. Dróżdż J, Demiaszkiewicz AW, Lachowicz J. 2003. Expansion of the Asiatic parasite Ashworthius sidemi (Nematoda, Trichostrongylidae) in wild ruminants in Polish territory. Parasitology Research, 89,
  10. Dróżdż J, Demiaszkiewicz AW, Lachowicz J. 2004. Endoparasites of the beaver Castor fiber (L.) in northeast Poland.  Helminthologia, 41, 99-101.
  11. Lotfy W.M, Brant S, DeJong RJ, Le T.H, Demiaszkiewicz A, Rajapakse RPWJ, Perera VBVP, Laursen JR, Loker ES. 2008. Evolutionary origins, diversification and biogeography of liver flukes (Digenea, Fasciolidae). American Journal of Tropical Medicine and Hygiene, 79, 248-255.
  12. Demiaszkiewicz AW, Kuligowska I, Lachowicz J, Osińska B. 2008. Experimental infection of Vietnam pigs with invasive Elaphostrongylus cervi larvae. Medycyna Weterynaryjna, 64, 815-817 (in Polish).
  13. Demiaszkiewicz AW, Lachowicz J, Osińska B. 2009. Ashworthius sidemi (Nematoda, Trichostrongylidae) in wild ruminants in Białowieża Forest. Polish Journal of Veterinary Sciences, 12, 385-388.
  14. Demiaszkiewicz AW, Polańczyk G, Pyziel AM, Kuligowska I, Lachowicz J.2009. The first loci of dirofilariosis of dogs evoked by Dirofilaria repens Railliet et Henry, 1911 in central Poland. Wiadomości Parazytologiczne, 55, 367-370 (in Polish).
  15. Osińska B, Demiaszkiewicz AW, Lachowicz J. 2010. Pathological lesions in European bison (Bison bonasus) with infestation by Ashworthius sidemi(Nematoda, Trichostrongylidae). Polish Journal of Veterinary Sciences,13, 63-67.
  16. Radwan J, Demiaszkiewicz AW, Kowalczyk R, Lachowicz J, Kawałko A, Wójcik JM, Pyziel AM, Babik W. 2010. An evaluation of two potential risk factors, MHC diversity and host density, for infection by an invasive nematode Ashworthius sidemi in endangered European bison (Bison bonasus). Biological Conservation, 143, 2049-2053.
  17. Pyziel AM., Kowalczyk R, Demiaszkiewicz AW. 2011. The annual cycle of shedding Eimeria oocysts by European bison (Bison bonasus) in the Bialowieza Primeval Forest. Journal of Parasitology, 97, 737-739.
  18. Demiaszkiewicz AW, Polańczyk G, Osińska B, Pyziel AM, Kuligowska I, Lachowicz J. 2011. Morphometric characteristics of Dirofilaria repens Railliet et Henry, 1911, parasite of dogs in Poland. Wiadomości Parazytologiczne, 57, 253-256.
  19. Demiaszkiewicz AW, Pyziel AM, Kuligowska I, Lachowicz J, Krzysiak MK. 2012. Nematodes of the large intestine of the European bison of the Białowieża National Park, Annals of Parasitology, 58, 9-13.
  20. Walker SM, Demiaszkiewicz AW, Kozak M, Wędrychowicz H, Teofanova D, Prodohl P, Brennan G, Fairweather I, Hoey EM, Trudgett A. 2012. Mitochondrial DNA haplotype analysis of liver fluke in bison from Bialowieza Primaeval Forest indicates domestic cattle as the likely source of infection. Veterinary Parasitology, http://dx.doi.org10.1016vetpar.2012.08.002p;
  21. Demiaszkiewicz AW, Karamon J, Jasik A. 2013. Przypadek wykrycia nicienia Dirofilaria repens w jądrze psa. Medycyna Weterynaryjna, 69, 124-127.
  22. Demiaszkiewicz AW, Kuligowska I, Lachowicz J, Pyziel AM, Moskwa B.2013. The first detection of nematodes Ashworthius sidemi in elk Alces alces (L.) in Poland and remarks of limits of ashworthiosis foci. Acta Parasitologica, 58, 515-518.
  23. Kuligowska I., Demiaszkiewicz AW.2013. Występowanie nicieni Elaphostrongylus cervi u jeleni na terenie Polski. Medycyna Weterynaryjna, 69, 630-663.
  24. Pyziel AM, Demiaszkiewicz AW.2013. Coccidia (Apicomplexa: Eimeriidae) of elk (Alces alces) in Poland. Parasitology Research, 112, 2083-2085.
  25. Demiaszkiewicz AW, Lachowicz J, Kuligowska I, Pyziel AM, Bełżecki G, Miltko R, Kowalik B, Gogola W, Giżejewski Z.2014. Parasitofauna of the European Beaver Castor fiber L. 1758 in north-eastern Poland. Bulletin of the Veterinary Institute in Pulawy, 58, 223-227.
  26. Demiaszkiewicz AW, Polańczyk G, Osińska B, Pyziel AM, Kuligowska I, Lachowicz J, Sikorski A.2014. Prevalence and distribution of Dirofilaria repens Railliet et Henry, 1911 in dogs in Poland. Polish Journal of Veterinary Sciences, 17, 515-517.
  27. Demiaszkiewicz AW, Pyziel AM, Kuligowska I, Lachowicz J.2014. The first report of Angiostrongylus vasorum (Nematoda: Metastrongyloidea) in Poland, in red foxes (Vulpes vulpes). Acta Paraitologica, 59, 758-762.
  28. Odojevskaya IM, Aseev V, Movsesyan SO, Voronin MV, Panayotova-Pencheva M, Petkova S, Dilcheva V, Demiaszkiewicz A.2014. Ecological and bilogical peculiarities and adaptation properties of European isolate of Thichinella nativa. Comptes Rendus de l`Academie Bulgare des Sciences, 67, 49-56.
  29. Osińska B, Demiaszkiewicz AW, Pyziel AM, Kuligowska I, Lachowicz J, Dolka I.2014. Prevalence of Dirofilaria repens in dogs in central-eastern Poland and histopathological changes caused by this infection. Bulletin of the Veterinary Institute in Pulawy, 58, 35-39.
  30. Pyziel AM.2014. Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU). Acta Parasitologica, 59, 122-125.
  31. Pyziel AM, Jóźwikowski M, Demiaszkiewicz AW.2014. Coccidia (Apicomplexa: Eimeriidae) of the lowland European bison (Bison bonasus bonasus L.) in Poland. Veterinary Parasitology, 202, 138-144.
  32. Pyziel AM, Demiaszkiewicz AW, Kuligowska I.2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from South-Western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523-525.
  33. Demiaszkiewicz AW.2014. Migrations and introduction of wild ruminants as a source of parasite exchange and emergence of new parasitoses. Annals of Parasitology, 60, 25-30.
  34. Demiaszkiewicz AW.2014. Dirofilaria repens Railliet et Henry, 1911 – a new parasite acclimatized in Poland. Annals of Parasitology, 60, 31-35.
  35. Demiaszkiewicz AW, Pyziel AM, Kuligowska I, Lachowicz J.2014. Dirofilariosis – a new parasitic disease of dogs and humans in Poland. Annual Report Polish Academy of Sciences 2014, 47-49.
  36. Demiaszkiewicz AW, Kuligowska I, Pyziel AM, Lachowicz J.2015. Pierwsze w Polsce przypadki inwazji nicieni Setaria tundra u łosi (Alces alces). Medycyna Weterynaryjna, 71, 510-512.
  37. Králová-Hromadová I, Bazsalovicsová E, Demiaszkiewicz AW.2015. Molecular characterisation of Fascioloides magna (Trematoda: Fascioloidae) from south-western Poland based on mitochondrial markers. Acta Parasitologica, 60, 544-547.
  38. Krzysiak MK, Demiaszkiewicz AW, Pyziel AM, Laska M.2015. Monitoring parazytologiczny żubrów Bison bonasus w rezerwatach hodowlanych Białowieskiego Parku Narodowego. Medycyna Weterynaryjna, 71, 791-795.
  39. Pyziel AM, Demiaszkiewicz AW.2015. Observations on sporulation of Eimeria bovis (Apicomplexa: Eimeriidae) from the European bison Bison bonasus (L.): effect of temperature and potassium dichromate solution. Folia Parasitologica, 61, 020.
  40. Pyziel AM, Laskowski Z, Höglund J.2015. Develompment of multiplex PCR for identification of Dictyocaulus lungworms in domestic and wild rumiants. Parasitology Research, 114-3923-3926.
  41. Demiaszkiewicz AW. Kuligowska I, Pyziel AM, Lachowicz J.2015. Extension of occurrence area of the American fluke Fascioloides magna in south-western Poland. Annals of Parasitology, 61, 93-96.
  42. Kołodziej-Sobocińska M, Pyziel AM, Demiaszkiewicz AW, Borowik T, Kowalczyk R. 2016. Pattern of parasite egg shedding by European bison (Bison bonasus) in the Białowieża Primeval Forest, Poland. Mammal Research, 61, 179-186.
  43. Demiaszkiewicz AW, Pyziel AM, Kuligowska I, Lachowicz J. 2016. Fascioloides magna, a parasite of red deer in Lower Silesian Wilderness. Medycyna Weterynaryjna, 72, 110-112.
  44. Pyziel AM, Jeżewski W. 2016. Coprology of a single Northern white-breasted hedgehog (Erinaceus roumanicus): first report of Isospora rastegaievae in Poland. Acta Parasitologica , 61, 636-638.