Laboratories

Prof. dr hab. Grzegorz Karbowiak - UNIT HEAD

Prof. dr hab. Zdzisław Świderski
Dr hab. Zdzisław Laskowski prof. nadzw. IP PAN
Dr hab. Mykola Ovcharenko prof. nadzw. IP PAN
Dr hab. Anna Rocka, prof. nadzw. IP PAN
Dr Witold Jeżewski
Dr Joanna Werszko
Dr Piotr Wróblewski
Mgr Anna Myczka
Mgr Tomasz Szewczyk



Research profile: general description:

The research are conducted on the biology, morphology and ultrastructure of blood parasites as Trypanosoma, Babesia, Hepatozoon and Bartonella in wild mammals, as well as the biology of ticks and fleas as the transmitters of these pathogens. The role of small rodents and ticks as a reservoir and transmitters of human and domestic zoonoses is important. Due to that the structure of outbreaks of transmission diseases and the relationships between small rodents and ticks is studied.

The Department also conducts research on the parasitological consequences of biological invasions on the example of bipedal crustacean parasites and goby family fish. The comparative analysis of the development cycles and ultrastructure of microsporidias parasitic in bipedal crustaceans (Gammaridae) is also examined.

Based on the specific molecular markers diagnostic tests are developed. This tests are helpfull to determine the species identification of parasites located in host tissues, regardless of the their developmental forms. These tests are also used in the identification of insect parasites, molluscs, terrestrial crustaceans and free living mammals. Additionally, adult and larval forms of tapeworms and nematodes, Antarctic vertebrate parasites are of particular interest. The study using molecular biology methods shed new light on the current systematics and biology of Antarctic parasites.

Extensive ultrastructural and cytochemical studies of embryogenesis of flukes (Trematoda) and tapeworms (Cestoda), their post-embryonic development and ultrastructure of invasive larval stages are also been conducted. In parallel, molecular studies of these parasitic flatworms are carried out to analyze their phylogeny, evolution and systematics.



Main research areas:

  1. Biology, systematics and zoogeography of helminthes (head: dr hab. Zdzisław Laskowski; staff: dr hab. Anna Rocka, dr. Witold Jeżewski, mgr Anna Myczka)
  2. Unicellular parasites (head: prof. dr hab. Grzegorz Karbowiak, staff: dr. Hab. Mykola Ovcharenko, dr Joanna Werszko, dr Piotr Wróblewski, mgr Tomasz Szewczyk)
  3. Biology of parasite development and reproduction (head: prof. dr hab. Zdzisław Świderski)

Current research activities:

  • Microparasites in water bodies subject to biological invasion (head: dr hab. M. Ovcharenko);
  • Microparasites of vertebrate blood and their vectors (head: prof. dr hab. G. Karbowiak);
  • Molecular diagnostics of selected sporores and bacteria in wild animals (head: dr hab. Z. Laskowski);
  • Developmental larval forms of parasites (nematodes, tapeworms, flukes and thornheads) in invertebrates of selected areas on Poliand (head: dr W. Jeżewski);
  • Domestic animals as the Blastocystis hominis reservoir (head: dr hab. Rocka);
  • Phylogenetic aspect of the functional ultrastructure of development stages of flukes and tapeworms (head: prof. dr hab. Z. Świderski)

Recent publications:

2019:

  1. Bojko J., Ovcharenko M. 2019. Pathogens and other symbionts of the Amphipoda: a review of their taxonomic diversity and pathological significance. Diseases of Aquatic Organisms, 136, 3-36, DOI: 10.3354/dao03321 – 100 pkt.
  2. Filip K.J., Pyziel A.M., Jeżewski W., Myczka A., Demiaszkiewicz A.W., Laskowski Z. 2019. First molecular identification of Taenia hydatigena in in wild ungulates in Poland. Eco Health,16, 1, 161-170, DOI: 10.1007/s10393-019-01392-9 – 70 pkt.
  3. Hildebrand J., Pyrka E., Sitko J., Jeżewski W., Zaleśny G., Tkach V., Laskowski Z. 2019. Molecular phylogeny provides new insights on the taxonomy and composition of Lyperosomum Looss, 1899 (Digenea, Dicrocoeliidae) and related genera. International Journal for Parasitology: Parasites and Wildlife. 9, 90-99. DOI: 10.1016/j.ijppaw.2019.03.020 – 100 pkt.
  4. Karbowiak G., Miklisova D., Stanko M., Werszko J., Hajdul-Marwicz M., Szewczyk T., Rychlik L. 2019 The competition between immatures of Ixodes ricinus and Dermacentor reticulatus (Ixodida: Ixodidae (ticks for rodent hosts. Journal of Medical Entomology, 56 (2), 448-452, doi: 10.1093/jme/tjy188 – 70 pkt.
  5. Kowalec M., Szewczyk T., Welc-Falęciak R., Siński E., Karbowiak G., Bajer A. 2019 Rickettsiales occurrence and co-occurrence in Ixodes ricinus ticks in natural and urban areas. Microbial Ecology, 77. 890-904, https://doi.org/10.1007/s00248-018-1269-y - 100 pkt.
  6. Pyziel A.M., Demiaszkiewicz A.W., Klich D., Laskowski Z. 2019. A morphological and molecular comparison of Eimeria bovis – like oocysts (Apicomplexa: Eimeriidae) from European bison, Bison bonasus L., and cattle, Bos taurus L. and the development of two multiplex PCR assays for their identification. Veterinary Parasitology, 275, 108917, DOI: 10.1016/j.vetpar.2019.08.011 – 140 pkt.
  7. Rocka A. 2019. Cestodes of the genus Tetrabothrius (Tetrabothridea: Tetrabothriidae) from Antarctic flying seabrids. Annals of Parasitology, 65, 423-426, doi:10.17420/ap6504.230 0 40 pkt.
  8. Szewczyk T., Werszko J., Myczka A.W., Laskowski Z., Karbowiak G. 2019. Molecular detection of Anaplasma phagocytophilum in wild carnivores in north-eastern Poland. Parasites and Vectors 12, 465. DOI: 10.1186/s13071-019-3734-y – 100 pkt.
  9. Werszko J., Szewczyk T., Steiner-Bogdaszewska Ż., Laskowski Z., Karbowiak G. 2019. Molecular detection of Anaplasma phagocytophilum in blood-sucking flies (Diptera: Tabanidae) in Poland. Journal of Medical Entomology, 56, 3, 822–827, DOI: 10.1093/jme/tjy217 – 70 pkt.
  10. Werszko J., Szewczyk T., Steiner-Bogdaszewska Ż., Wróblewski P., Karbowiak G., Laskowski Z. 2019. Molecular detection of Megatrypanum trypanosomes in tabanid flies. Medical and Veterinary Entomology, 2019 Sep 30, 1365-2915, DOI: 10.1111/mve.12409 – 100 pkt.

2018:

  1. Bacela-Spychalska K., Wróblewski P., Mamos T., Grabowski M., Rigaud T., Wattier R., Rewicz T., Konopacka A., Ovcharenko M. 2018. Europe-wide reassessment of Dictyocoela (Microsporidia) infecting native and invasive amphipods (Crustacea): molecular versus ultrastructural traits. Scientific Reports, 8: 8945. IF- 4.122
  2. Conn D.B., Świderski Z., Miquel J. 2018. Ultrastructure of digenean trematode eggs (Platyhelminthes: Neoophora): A review emphasizing new comparative data on four European Microphalloidea. Acta Parasitologica, 63, 1-14. IF-1.039
  3. Cybulska A., Skopek R., Kornacka A., Popiołek M., Piróg A., Laskowski Z., Moskwa B. 2018. First detection of Trichinella pseudospiralis infection in raccoon (Procyon lotor) in Central Europe. Veterinary Parasitology, 254, 114-119. IF-2.422
  4. Kuzmina T.A., Tkach V.V., Spraker T.R., Lyons E.T., Kudlai O. 2018. Digeneans of northern fur seals Callorhinus ursinus (Pinnipedia: Otariidae) from five subpopulations on St. Paul Island, Alaska. Parasitology Research, 117, 1079-1086. IF-2.558
  5. Pyziel A.M., Laskowski Z., Höglund J. 2018. An assessment of the use of cox1 and cox3 mitochondrial genetic markers for the identification of Dictyocaulus spp. (Nematoda: Trichostrongyloidea) in wild ruminants. Parasitology Research, 117, 2341-2345. IF-2.558
  6. Pyziel A.M., Dolka I., Werszko J., Laskowski Z., Steiner-Bogdaszewska Ż., Wiśniewski J., Demiaszkiewicz A.W., Anusz K. 2018. Pathological lesions in the lungs of red deer Cervus elaphus (L.) induced by a newly-described Dictyocaulus. Veterinary Parasitology, 261, 22-26. IF-2.422
  7. Solarczyk P., Wojtkowiak-Giera A., Holysz M., Słodkowicz-Kowalska A., Jagodzinski P., Stojecki K., Rocka A., Majewska A.C., Skrzypczak Ł. 2018. New Primers for fast detection of Giardia duodenalis assemblages A and B using Real-time PCR. Acta Protozoologica, 57, 43-48. IF-1.038
  8. Świderski Z., Miquel J., Azzouz-Maache S., Pétavy A.F. 2018. Echinococcus multilocularis (Cestoda, Cyclophyllidea, Taeniidae): functional ultrastructure of the penetration glands and nerve cells within the oncosphere. Parasitology Research, 117, 2653-2663. IF-2.558
  9. Świderski Z., Miquel J., Azzouz-Maache S., Pétavy A.F. 2018. Echinococcus multilocularis (Cestoda, Cyclophyllidea, Taeniidae): origin, differentiation and functional ultrastructure of the oncospheral tegument and hook region membrane. Parasitology Research, 117,783-791. IF-2.558
  10. Tkach V.V., Kinsella J.M., Greiman S.E. 2018. Two new species of Staphylocystoides Yamaguti, 1959 (Cyclophyllidea: Hymenolepididae) from the Masked Shrew Sorex cinereus in North America. Journal of Parasitology, 104,157-167. IF=1.395

2017:

  1. Kloch A., Mierzejewska E., Karbowiak G., Slivinska K., Alsarraf M., Rodo A., Kowalec M., Dwuznik D., Didyk Y., Bajer A. 2017. Origins of recently emerged foci of the tick Dermacentor reticulatus in central Europe inferred from molecular markers. Veterinary Parasitology, 237, 63-69. IF-2.356
  2. Kowalec M., Szewczyk T., Welc-Faleciak R., Siński E., Karbowiak G., Bajer A. 2017. Ticks and the city - are there any differences between city parks and natural forests in terms of tick abundance and prevalence of spirochaetes? Parasites & Vectors 10:573. IF-3.036
  3. Miquel J., Świderski Z., Sripa B., Ribas A. 2017. Ultrastructural characters of the spermatozoon of the liver fluke Opisthorchis viverrini (Poirier, 1886) (Opisthorchiidae). Parasitology Research 116, 2499-2506. IF-2.329
  4. Ovcharenko M., Wróblewski P., Kvach Y., Drobiniak O.2017. Study of Loma acerinae (Microsporidia) detected from three Ponto-Caspian gobies (Gobiidae) in Ukraine. Parasitology Research, 116, 1453-1462. IF-2.329
  5. Pyziel A.M., Laskowski Z., Demiaszkiewicz A.W., Hoglund J. 2017. Phylogeny of Dictyocaulus spp. in wild ruminants with morphological description of Dictyocaulus cervi n. sp. (Nematoda: Trichostrongyloidea) from red deer, Cervus elaphus. Journal of Parasitology 105, 506-518. IF-1.326
  6. Szewczyk T., Werszko J., Steiner-Bogdaszewska Ż., Jeżewski W., Laskowski Z., Karbowiak G. 2017. Molecular detection of Bartonella spp. in deer ked (Lipoptena cervi) in Poland. Parasite & Vectors 10:487, 1-7. IF-3.036
  7. Świderski Z, Miquel J, Azzouz-Maache S, Pétavy AF. 2017. Origin, differentiation and functional ultrastructure of egg envelopes in the cestode Echinococcus multilocularis Leuckart, 1863 (Cyclophyllidea: Taeniidae). Parasitology Research, 116, 1963-1971. IF-2.329

Prof. dr hab. Bożena Moskwa - UNIT HEAD

Prof. dr hab. Władysław Cabaj
Prof. dr hab. Aleksander W. Demiaszkiewicz
Dr hab. Justyna Bień-Kalinowska
Dr hab. Jakub Gawor
Mgr Żaneta Steiner-Bogdaszewska
Mgr inż. Marek Bogdaszewski
Mgr Aleksandra Cybulska
Mgr Aleksandra Kornacka
Lek. wet. Katarzyna Filip-Hutsch
Lek wet. Aneta Gralak
Teresa Sitek – senior technician



Research profile: general description:

Research is carried out on the parasites of free-living and domestic animals, their spread, morphology, systematics, diagnosistics and biology, as well as on parasitic diseases caused by them, their epizootiology, pathology and treatment. The potential risk of infection for humans and animals, as well as the pathways of parasite transmission are also assessed.

The protozoa of the Sarcocystidae family are of particular interest: Neospora caninum, Toxoplasma gondii, Sarcocystis spp., Trichinella nematodes, gastrointestinal nematodes of the Trichostrongylidae family (including blood-bearing Ashworthius sidemi), lung nematodes of the Protostrongylidae, tissue nematodes from the subfamily Onchocercinae and flukes in the family Fasciolidae (Parafasciolopsis fasciolaemorpha and Fascioloides magna).

Genotyping of parasite isolates obtained from various hosts based on selected nucleotide sequences is of great importance for the conducted research. It is possible to show possible phylogenetic differences between isolates obtained from various hosts from Poland and isolates from other countries and to compare the obtained results with the sequences deposited in GenBank.

An important research scope is the searching for potential markers for the the parasite diagnosis and immunoprotection against the infection of Trichinella nematodes in humans. Native and recombinant parasite proteins are used.



Main research areas:

  1. Physiopathology in the parasite-host system (head: prof. Bożena Moskwa; Staff: prof. Władysław Cabaj, dr hab. Justyna Bień-Kalinowska, dr Anna Stachyra, MSc Aleksandra Cybulska, MSc Aleksandra Kornacka, MSc Marek Bogdaszewski, MSc Żaneta Steiner-Bogdaszewska, MSc Sylwia Grzelak)
  2. Parasitic invasions in wild and domestic animals (head: prof. Aleksander W. Demiaszkiewicz; Staff: dr hab. Jakub Gawor, lek vet Katarzyna Filip-Hutsch, lek vet Aneta Gralak, Teresa Sitek - senior technician)

Current research activities:

  • Molecular diagnostics of pulmonary nematode species (Metastrongyloidea, Protostrongylidae) in chamois (Rupicapra rupicapra tatrica) in the Tatra National Park (head: dr hab. Jakub Gawor );
  • Parasites and parasitic diseases of wild ruminants ( head: prof. dr hab. Aleksander W. Demiaszkiewicz);
  • Occurrence and species identification of selected parasites in free-living animals in Poland (head: prof. dr hab. B. Moskwa);
  • Sarcocystis spp. in deer living in farm conditions and in an open environment (prof. dr hab. Władysław Cabaj);
  • Physiological and behavioral aspects of Cervidae farming (Msc M. Bogdaszewski).

Recent publications:

2019:

  1. Cybulska A., Kornacka A., Moskwa B. 2019. The occurrence and muscle distribution of Trichinella britovi in raccoon dogs (Nyctereutes procyonoides) in wildlife in the Głęboki Bród Forest District, Poland. International Journal for Parasitology: Parasites and Wildlife, 9, 149-153, DOI:10.1016/j.ijppaw.2019.05.003 - 100 pkt.
  2. Filip K.J., Pyziel A.M., Jeżewski W., Myczka A., Demiaszkiewicz A.W., Laskowski Z. 2019. First molecular identification of Taenia hydatigena in in wild ungulates in Poland. Eco Health,16, 1, 161-170, DOI: 10.1007/s10393-019-01392-9 – 70 pkt.
  3. Filip-Hutsch K., Hutsch T., Kolasa S., Demiaszkiewicz A.W. 2019. First description of histopathological lesions associated with fatal infection of moose (Alces alces) with liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932. Journal of Veterinary Research, 63, 4, 549-554, DOI: 10.2478/jvetres-2019-0068 – 40 pkt.
  4. Gawor J. 2019. Tasiemiec księżnej pani - prawdziwe i wydumane pasożytnicze zagrożenia odzwierzęce. Magazyn Weterynaryjny, 28, 34-39.
  5. Grzybek M., Cybulska A., Tołkacz K., Alsarraf M., Behnke-Borowczyk J., Szczepaniak K., Strachecka A., Paleolog J., Moskwa B., Behnke J.M., Bajer A. 2019. Seroprevalence of Trichinella spp. infection in bank voles (Myodes glareolus) – A long term study. International Journal for Parasitology: Parasite and Wildlife, 9, 144-148; DOI: 10.1016/j.ijppaw.2019.03.005 - 100 pkt.
  6. Laurimäe T., Kinkar L., Romig T., Umhang G., Casulli A., Omer R.A., Sharbatkhori M., Mirhendi H., Ponce-Gordo F., Lazzarini L., Viviana Soriano S., Varcasia A., Rostami- Nejad M., Andresiuk V., Maravilla P., Miguel Gonzalez L., Dybicz M., Gawor J., Šarkunas M., Šnabel V., Kuzmina T., Beigom Kia E., Saarma U. 2019. Analysis of nad2 and nad5 enables reliable identification of genotypes G6 and G7 within the species complex Echinococcus granulosus sensu lato. Infection, Genetics et Evolution, 74, 103941, doi.org/10.1016/j.meegid.2019.103941. – 100 pkt.
  7. Miltko R., Kowalik B., Kędzierska A., Demiaszkiewicz A.W., McEwan N.R., Obidziński A., Bełżecki G. 2019. Effect of seasonal diet composition changes on the characteristics of gastrointestinal tract contents of Eurasian beaver (Castor fiber). Journal of Animal and Feed Sciences, 28, 392-397, DOI: 10.22358/jafs/114435/2019 - 40 pkt.
  8. Pyziel A.M., Demiaszkiewicz A.W., Klich D., Laskowski Z. 2019. A morphological and molecular comparison of Eimeria bovis – like oocysts (Apicomplexa: Eimeriidae) from European bison, Bison bonasus L., and cattle, Bos taurus L. and the development of two multiplex PCR assays for their identification. Veterinary Parasitology, 275, 108917, DOI: 10.1016/j.vetpar.2019.08.011 – 140 pkt.
  9. Stachyra A., Zawistowska-Deniziak A., Basałaj K., Grzelak S., Gondek M., Bień-Kalinowska J. 2019. The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella britovi Produced in Yeast. Frontiers in Immunology, 10, 2420. DOI:10.3389/fimmu.2019.02420. eCollection 2019 - 140 pkt.
  10. Tajchman K., Bogdaszewski M., Kowalczuk-Vasilew E. 2019. Effects of supplementation with different levels of Ca and P on mineral conent of first antler, bone, muscle and liver of farmed fallow deer (Dama dama). Candian Journal of Animal Science, DOI: 10.1139/CJAS-2018-0234 – 40 pkt.
  11. Tajchman K., Bogdaszewski M., Kowalczuk-Vasilew E., Dąbrowski R. Impact of day length and total protein content in the diet of farmed fallow deer (Dama dama) on their plasma mineral level and haematological indices. 2019. Applied Ecology and Environmental Research 17 (6), 14729-14750, DOI: http://dx.doi.org/10.15666/aeer/1706-1472914750 - 40 pkt.
  12. Tajchman K., Steiner-Bogdaszewska Ż., Kowalczuk-Vasilev E., Dąbrowski R. 2019. Effect of Ca and P supplementation on the haematological parameters and content of selected minerals in the blood of young farmed fallow deer males (Dama dama). Biologia, 1–11 - 40 pkt.
  13. Werszko J., Szewczyk T., Steiner-Bogdaszewska Ż. , Laskowski Z., Karbowiak G. 2019. Molecular detection of Anaplasma phagocytophilum in blood-sucking flies (Diptera: Tabanidae) in Poland. Journal of Medical Entomology, 56, 3, 822–827, DOI: 10.1093/jme/tjy217 – 70 pkt.
  14. Werszko J., Szewczyk T., Steiner-Bogdaszewska Ż. , Wróblewski P., Karbowiak G., Laskowski Z. 2019. Molecular detection of Megatrypanum trypanosomes in tabanid flies. Medical and Veterinary Entomology, 2019 Sep 30, 1365-2915, DOI: 10.1111/mve.12409 – 100 pkt.

2018:

  1. Bełżecki G., Miltko R., Kowalik B., Demiaszkiewicz A.W. , Lachowicz J., Giżejewski Z., Obidzinski A., McEwan N.R. 2018. Seasonal variations of the digestive tract of the Eurasian beaver Castor fiber. Mammal Research, 63, 21-31. IF-1.299
  2. Cybulska, A. Skopek, R., Kornacka A. , Popiolek M., Pirog A., Laskowski Z., Moskwa B. 2018. First detection of Trichinella pseudospiralis infection in raccoon (Procyon lotor) in Central Europe. Veterinary Parasitology, 254, 114-119. IF-2.422
  3. Demiaszkiewicz A. , Bielecki W., Rodo A., Pyziel A.M. , Filip K.J. 2018. Parasitofauna of European bison Bison bonasus (L.) in Borecka Forest. Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 74, 253-256. IF- 0.197
  4. Demiaszkiewicz A. , Kowalczyk R., Filip K. , Pyziel A.M. 2018. Fascioloides magna: a parasite of roe deer in Bory Zielonogórskie. Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 74, 257-260. IF- 0.197
  5. Gondek M., Bień J. , Nowakowski Z. 2018. Use of ELISA and Western blot for serological detection of antibodies to E-S antigens of Trichinella spiralis muscle larvae in sera of swine experimentally infected with Trichinella spiralis. Veterinary Immunology and Immunopathology, 203, 13-20. IF-1.632
  6. Kołodziej-Sobocińska M., Demiaszkiewicz A.W. , Pyziel A.M. , Kowalczyk R. 2018. Increased parasitic load in European bison (Bison bonasus) released from captivity - implications for reintroduction programs. EcoHealth, 15, 467-471. IF-2,649
  7. Kornacka A. , Cybulska A. , Moskwa B. 2018. Comparison of sensitivity of two premers sets for the detection of Toxoplasma gondii DNA in wildlife. Acta Parasitologica, 63, 635-640. IF-1.039
  8. Moskwa B. , Kornacka A. , Cybulska A. , Cabaj W. , Reiterova K., Bogdaszewski M. , Steiner-Bogdaszewska Ż. , Bień J. 2018. Seroprevalence of Toxoplasma gondii and Neospora caninum infection in sheep, goats and fallow deer farmed on the same area. 2018. Journal of Animal Sience, 96, 2468-2473. IF-1.711
  9. Pyziel A. , Bjorck S., Wiklund R., Skarin M., Demiaszkiewicz A. , Hoglund J. 2018. Gastrointestinal parasites of captive European bison Bison bonasus (L.) with a sign of reduced efficacy of Haemonchus contortus to fenbendazole. Parasitology Research, 117, 295-302. IF-2.558
  10. Pyziel A.M. , Dolka I., Werszko J., Laskowski Z., Steiner-Bogdaszewska Ż. , Wiśniewski J., Demiaszkiewicz A.W. , Anusz K. 2018. Pathological lesions in the lungs of red deer Cervus elaphus (L.) induced by a newly-described Dictyocaulus. Veterinary Parasitology, 261, 22-26. IF-2.422
  11. Sulima A., Savijoki K., Bień J. , Näreaho A., Sałamatin R., Conn D.B., Młocicki D. 2018. Comparative Proteomic Analysis of Hymenolepis diminuta Cysticercoid and Adult Stages. Frontiers in Microbiology, 15;8:2672. IF-4.019

2017:

  1. Cabaj W. , Bień J. , Bogdaszewski M. , Steiner-Bogdaszewska Ż. , Moskwa B. Potential impact of Neospora caninum infection on farm productivity of fallow deer (Dama dama). Small Ruminants Research, 156, 78-81. IF-0.947
  2. Demiaszkiewicz A.W. , Merta D., Kobielski J., Pyziel A.M. , Filip K.J. 2017. Expansion of Ashworthius sidemi in red deer and roe deer from the Lower Silesian Wilderness and its impact on infection with other gastrointestinal nematodes. Acta Parasitologica, 62, 853-857. IF-1.160
  3. Gawor J. , Borecka A. 2017. Quantifying the risk of zoonotic geohelminth infections for rural household inhabitants in central Poland. Ann. Agric. Environ. Med. 24, 44-48.
  4. Gawor J. , Gromadka R., Zwijacz-Kozica T., Zięba F. 2017. A modified method for molecular identification of Baylisascaris transfuga in European brown bears (Ursus arctos). Parasitology Research, 116, 3447-3452. IF-2.329
  5. Gondek M., Bień J. , Nowakowski Z. 2017. Detection of experimental swine trichinellosis using commercial ELISA test. Polish Journal of Veterinary Sciences, 20, 445-454. IF- 0.697
  6. Pyziel A.M. , Laskowski Z., Demiaszkiewicz A.W. , Hoglund J. 2017. Phylogeny of Dictyocaulus spp. in wild ruminants with morphological description of Dictyocaulus cervi n. sp. (Nematoda: Trichostrongyloidea) from red deer, Cervus elaphus. Journal of Parasitology, 105, 506-518. IF- 1.326
  7. Sałamatin R., Kowal J., Nosal P., Kornaś S., Cielecka D., Jańczak D., Patkowski4 W., Gawor J., Kornyushin V., Gołąb E., Šnábel V. 2017. Cystic echinococcosis in Poland: genetic variability and the first record of Echinococcus granulosus sensu stricto (G1 genotype) in the country. Parasitology Research, 116, 3077-3085. IF-2.329
  8. Goździk K., Odoevskaya I.M., Movsesyan S.O., Cabaj W. 2017. Molecular identification of Trichinella isolates from wildlife animals of the Russian Arctic territories; a new host record. Helminthologia, 54, 11-16. IF-0.609
  9. Laurimäe T., Kinkar L., Romig T., Omer R.A., Casullid A., Umhang G., Gasser R.B., Jabbar A., Sharbatkhori M., Mirhendi H., Ponce-Gordo F., Lazzarini L.J., Soriano S.V., Varcasia A., Nejad M.R., Andresiuk V., Maravilla P., González L.M., Dybicz M., Gawor J., Šarkūnas M., Šnábel V., Kuzmina T., Saarma U. 2018. The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7. Infection, Genetics and Evolution, 64, 85-94. IF-2.545
  10. Łukasik I., Kornacka A., Goławska S., Sytykiewicz H., Sprawka I., Wójcicka A. 2017. Effects of Acyrtosiphon pisum (Harris) infestation on the hydrogen peroxide content and activity of antioxidant enzymes in Fabaceae plants. Allelopathy Journal, 40, 143-150. IF=1,050
  11. Moskwa B., Bień J., Kornacka A., Cybulska A., Goździk K., Krzysiak MK, Reiterova K, Cabaj W. 2017. First Toxoplasma gondii isolate from an aborted foetus of European bison (Bison bonasus bonasus L.). Parasitology Research, 116, 2457-2461. IF-2.329
  12. Sulima A., Bień J., Savijoki K., Näreaho A., Salamatin R., Conn D.B., Mlocicki D. 2017. Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta. Parasites & Vectors, 10(1):577. IF- 3.035

Prof. dr hab. Mieczysława Boguś - UNIT HEAD

Prof. dr hab. Halina Wędrychowicz
Dr hab. Daniel Młocicki, prof. nadzw. IP PAN
Dr inż. Katarzyna Basałaj
Dr inż. Anna Zawistowska-Deniziak
Dr inż. Agnieszka Wesołowska
Dr Michalina Kazek
Dr Emilia Włoka
Dr Anna Wrońska
Dr Agata Kaczmarek
Mgr inż. Alicja Kalinowska
Mgr Przemysław Wilkowski



Research profile: general description:

Topics related to the interaction between the parasite and host are examined: biochemical, immunological and molecular processes occurring in the parasite-host system, disease processes caused by the parasite and host defense responses. The research is carried out in two systems: entomopathogenic fungus (Conidiobolus coronatus) - insect and parasitic flatworm (Fasciola hepatica) - mammal.

Entomopathogenic fungi producing mycotoxins are of particular interest as a potential source of a new generation of insecticides. Due to that , the mechanisms of insect paralysis and their defensive reactions are examined in the Conidiobolus coronatus - insect research system. Identification and determination of the role of fungal metabolites in impaired functioning of the insect's immune system is of great importance. Demonstration of the presence of many substances produced by C. coronatus such as benzyl alcohol, octanoic acid, dodacanol, caprylic acid, harmine, nor-harmine, which participation in the infection has not been known so far, gives new research possibilities.

In the experimental system of Fasciola hepatica - mammal, studies are conducted on the immunoprevention of infection using native and recombinant proteins. The immunomodulatory properties of parasite proteins in interaction with mammalian macrophages, determining the effect of intestinal epithelial cells and their interaction with antigen presenting cells (APC) on induction of local immune response during parasitic invasion are also studied. Recent studies on the immunomodulatory properties of flatworm proteins (F. hepatica, Hymenolepis diminuta) indicate that they can be used to directly manipulate immune processes in the autoimmune diseases.



Main research areas:

  1. Molecular basis of immunoprevention of parasitic infection (Head: prof. dr hab. Halina Wędrychowicz; Staff: dr hab. Daniel Młocicki, dr Katarzyna Basałaj, dr Anna Zawistowska-Deniziak, dr Agnieszka Wesołowska, MSc Alicja Kalinowska, MSc Przemysław Wilkowski)
  2. Mechanisms of entomopathogenic fungi 's impact on insects (Head: prof. dr hab. Mieczysława Boguś; staff: dr Michalina Kazek, dr Emilia Włóka, dr Anna Wrońska, dr Agata Kaczmarek)

Current research activities:

  • The effect of infection with the entomopathogenic fungus Conidiobolus coronatus and the topical administration of its two metabolites (harman and norharman) on the level of selected markers of inflammation in the hemolymph of Galleria mellonella larvae (head: prof. dr hab. Mieczysława Boguś);
  • Immunoprevention of helminth infection (head: prof. dr hab. Halian Wędrychowicz);
  • Cloning and expression of helminth parasite proteins (head: prof. dr hab. Halina Wędrychowicz).

Recent publications:

2019:

  1. Bąska, P., Zawistowska-Deniziak, A., Norbury, L.J. , Wiśniewski, M., Januszkiewicz, K. 2019. Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages. Journal of Veterinary Research, 63, 1, 63 –70, https://doi.org/10.2478/jvetres-2019-0011 - 40 pkt.
  2. Buffoni L., Piva M.M., Bąska P., Januszkiewicz K., Norbury L.J. , Prior K.C., Dezen D., Silva A.S., Wedrychowicz H. , Mendes R.E. 2019. Immunization with the recombinant myosin regulatory light chain (FhrMRLC) in Adjulex® adjuvant elicits a Th1-biased immune response and a reduction of parasite burden in Fasciola hepatica infected rats. Parasitology International, DOI: https://doi.org/10.116/j.parint.2019.102037 - 70 pkt.
  3. Długosz, E., Basałaj, K. , Zawistowska-Deniziak, A. 2019. Cytokine production and signalling in human THP-1 macrophages is dependent on Toxocara canis glycans. Parasitology Research, 118, 10, 2925-2933 https://doi.org/10.1007/s00436-019-06405-8 -70 pkt.
  4. Kazek M. , Kaczmarek A. , Wrońska A.K. , Boguś M.I. 2019. Diet influences the bacterial and free fatty acid profiles of the cuticle of Galleria mellonella larvae. PloS ONE, 14, 2, e0211697. DOI:10.1371/journal.pone.0211697- 100 pkt.
  5. Norbury L. , Basałaj K. , Bąska P., Zawistowska-Deniziak A. , Kalinowska A. , Wilkowski P. , Wesołowska A. , Wędrychowicz H. 2019. Generation of a single-chain variable fragment phage display antibody library from naïve mice panned against Fasciola hepatica antigens. Experimental Parasitology 2019, 107737, Oct; 205:107737. https://doi.org/10.1016/j.exppara.2019.107737- 70 pkt.
  6. Norbury L.J. , Basałaj K. , Bąska P., Kalinowska A. , Zawistowska-Deniziak A. , Yap H.Y., Wilkowski P. , Wesołowska A. , Wędrychowicz H. 2019. Construction of a novel phage display antibody library against Fasciola hepatica, and generation of a single-chain variable fragment specific for F. hepatica cathepsin L1. Experimental Parasitology, 198, 87-94, https://doi.org/10.1016/j.exppara.2019.02.001 - 70 pkt.
  7. Nowak R.M., Jastrzębski J.P., Kuśmirek W., Sałamatin R., Rydzanicz M., Sobczyk-Kopcioł A., Sulima-Celińska A., Paukszto Ł., Makowczenko K.G., Płoski R., Tkach V.V., Basałaj K. , Młocicki D. 2019. Hybrid de novo whole-genome assembly and annotation of the model tapeworm Hymenolepis diminuta. Scientific Data, 6:302, https://doi.org/10.1038/s41597-o19-0311-3 - 140 pkt.
  8. Stachyra A., Zawistowska-Deniziak A. , Basałaj K. , Grzelak S., Gondek M., Bień-Kalinowska J. 2019. The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella britovi Produced in Yeast. Frontiers in Immunology, 10, 2420. DOI:10.3389/fimmu.2019.02420. eCollection 2019 - 140 pkt.
  9. van der Zande, H.J.P., Zawistowska-Deniziak A. , Guigas, B., 2019. Immune Regulati of Metabolic Homeostasis by Helminths and Their Molecules. Trends in Parasitology, 35, 10, 795- 808, https://doi.org/10.1016/j.pt.2019.07.014 - 140 pkt.
  10. Wesołowska A. , Basałaj K. , Zawistowska-Deniziak A. , Januszkiewicz K., Kozak Ljunggren M., Jedlina L., Wędrychowicz H. 2019. The failure of DNA prime/protein boost regime and CTLA-4 mediated targeting to improve the potency of DNA vaccine encoding Fasciola hepatica phosphoglycerate kinase in sheep. Veterinary Immunology and Immunopathology, 217, 109941. https://doi.org/10.1016/j.vetimm.2019.109941 - 70 pkt.
  11. Wrońska A.K. , Boguś M.I. 2019. Harman and norharman, metabolites of the entomopathogenic fungus Conidiobolus coronatus (Entomophthorales), affect the serotoninlevels and phagocytic activity of hemocytes, insect immunocompetent cells, in Galleria mellonella (Lepidoptera). Cell & Bioscience, 9, 1, 29. DOI: 10.1186/s13578-019-0291-1 – 100 pkt.
  12. Zielińska, D., Długosz, E., Zawistowska-Deniziak A. 2019. Functional Properties of Food Origin Lactobacillus in the Gastrointestinal Ecosystem - In Vitro Study. Probiotics and Antimicrobial Proteins, 11, 3, 820-829, https://doi.org/10.1007/s12602-018-9458-z -70 pkt.

2018:

  1. Cerkowniak M., Boguś M.I. , Włóka E. , Stepnowski P., Gołębiowski M. 2017. Application of headspace solid-phase microextraction followed by gas chromatography coupled with mass spectrometry to determine esters of carboxylic acids and other volatile compounds in Dermestes maculatus and Dermestes ater lipids. Biomedical Chromatography, 32, 1-13. IF- 1.688
  2. Norbury L.J. , Basałaj K. , Zawistowska-Deniziak A. , Sielicka A. , Wilkowski P. , Wesołowska A. , Smooker P.M., Wędrychowicz H. 2018. Intranasal delivery of a formulation containing stage-specific recombinant proteins of Fasciola hepatica cathepsin L5 and cathepsin B2 triggers an anti-fecundity effect and an adjuvant-mediated reduction in fluke burden in sheep. Veterinary Parasitology, 258, 14-23. IF-2.422
  3. Sulima A., Savijoki K., Bień J., Näreaho A., Sałamatin R., Conn D.B., Młocicki D. 2018. Comparative proteomic analysis of Hymenolepis diminuta cysticercoid and adult stages. Frontiers in Microbiology, 15, 8:2672. IF- 4.019
  4. Wrońska A.K. , Boguś M.I. , Włóka E. , Kazek M. , Kaczmarek A. , Zalewska K. 2018. Cuticular fatty acids of Galleria mellonella (Lepidoptera) inhibit fungal enzymatic activities of pathogenic Conidiobolus coronatus. PLoS One, 8;13(3):e0192715. IF- 2.766
  5. Wesołowska A. , Basałaj K. , Norbury L.J. , Sielicka A. , Wędrychowicz H. , Zawistowska-Deniziak A. 2018. Sex and vaccination: Insights from female rats vaccinated with juvenile-specific proteases from Fasciola hepatica. Veterinary Parasitology, 255, 91-96. IF-2.422
  6. Wesołowska A. , Basałaj K. , Norbury L.J. , Sielicka A. , Wędrychowicz H. , Zawistowska-Deniziak A. 2018. Vaccination against Fasciola hepatica using cathepsin L3 and B3 proteases delivered alone or in combination. Veterinary Parasitology, 250, 15-21. IF-2.422
  7. Wesołowska A. , Zawistowska-Deniziak A. , Norbury L.J. , Wilkowski P. , Pyziel A.M., Zygner W., Wędrychowicz H. 2018. Lymphocyte responses of rats vaccinated with cDNA encoding a phosphoglycerate kinase of Fasciola hepatica (FhPGK) and F. hepatica infection. Parasitology International, 67, 85-92. IF-2.055

2017:

  1. Bąska, P. Norbury, L.J., Zawistowska-Deniziak, A., Wiśniewski, M., Januszkiewicz, K. 2017. Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine “BOMA” macrophages. Parasitology Research, 31, 23-35. IF-2.329
  2. Boguś M.I. , Włóka E. , Wrońska A. , Kaczmarek A. , Kazek M. , Zalewska K., Ligęza-Żuber M. , Gołębiowski M. 2017. Cuticle hydrolysis in four medically important fly species by enzymes of the entomopathogenic fungus Conidiobolus coronatus. Medical and Veterinary Entomology, 16, 2775-2782. IF-1.809
  3. Cerkowniak M., Boguś M.I. , Włóka E. , Stepnowski P., Gołębiowski M. 2017. Comparison of the volatile compounds of Dermestes maculatus and Dermestes ater pupae: application of headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC/MS). Invertebrate Survival Journal, 14, 303-311. IF-0.824
  4. Henker L.C., Schwertz C.I., Lucca N.J., Piva M.M., Prior K.C., Baska P., Norbury L. , Januszkiewicz K. , Dezen D., Duarte M.M.M.F., Moresco R.N., Bertagnolli da Rosa L., Mendes R.E. 2017. Immune protection conferred by recombinant MRLC (myosin regulatory light chain) antigen in TiterMax Gold® adjuvant against experimental fasciolosis in rats.Vaccine 35, 663-671. IF-3.235
  5. Henker L.C., Schwertz C.I., Lucca N.J., Piva M.M., Giacomin P., Gris A., Rhoden L.A., Norbury L.J. , Da Silva A.S., Da Rosa, R.A., Mendes R.E. 2017. Dictyocaulosis in dairy cows in Brazil: An epidemiological, clinical-pathological and therapeutic approach. Acta Parasitologica, 62, 129-132. IF-1.116
  6. Kęsik-Brodacka M., Lipiec A., Kozak-Ljunggren M. , Jedlina L. , Miedzińska K., Mikołajczak M., Płucienniczak A., Legocki A.B., Wędrychowicz H. 2017. Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae. PLoS Neglected Tropical Diseases, 11(3),1-18, e0005451. IF-3.834
  7. Staniszewska M., Bondaryk, M., Kazek M. , Gliniewicz A., Braunsdorf C., Schaller M., Mora-Montes H.M., Ochal Z. 2017. Effect of serine protease KEX2 on Candida albicans virulence under halogenated methyl sulfones. Future Microbiology, 12, 285-306. IF-3.374
  8. Sulima A., Bień J., Savijoki K., Näreaho A., Sałamatin R., Conn D.B., Młocicki D. 2017. Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta. Parasite & Vectors, 10:577. IF-3.035
  9. Tarkowski W., Moneta-Wielgoś J., Młocicki D. 2017. Do Demodex mites play a role in pterygium development? Medical Hypotheses, 98, 6-10. IF-1.066
  10. Zawistowska-Deniziak A. , Basałaj K. , Strojny B., Młocicki D. 2017. New data on human macrophages polarization by Hymenolepis diminuta tapeworm-An in vitro study. Frontiers in Immunology, 8:148, 1-15. IF-6.429